Acute and chronic diarrhoea and abdominal colic associated with enteroaggregative Escherichia coli in young children living in western Europe
Acute and chronic diarrhoea and abdominal colic associated with enteroaggregative Escherichia coli in young children living in western Europe
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DOI：
10.1016/s0140-6736(96)12485-5
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发表时间：
1997-06-07
期刊：
影响因子：
168.9
通讯作者：
Karch, H
中科院分区：
文献类型：
--
作者：
Huppertz, HI;Rutkowski, S;Karch, H
肠聚集性大肠杆菌（EAggEC或EAEC）可在发展中国家传播并引起疾病，但目前尚不清楚它是否在工业化国家传播疾病。因此，我们进行了一项前瞻性研究，以评估德国儿童EAEC感染的发病率和临床表现。方法798例腹泻儿童，在24个月的时间内在一个确定的地理区域内住院，被纳入试验，EAEC培养粪便标本，PCR筛选，并通过菌落杂交从毒力质粒上发现的DNA序列鉴定，这些发现通过对HEp-2细胞的聚集粘附得到证实。粪便样本从580名儿童入院无腹泻controls.Findings EAEC被发现在粪便中的16（2%）的798名儿童腹泻，但没有在580名儿童没有腹泻，只有四个EAEC感染的儿童曾前往发展中国家。大多数EAEC感染发生在夏季。12例患儿感染EAEC后出现急性水样腹泻，4例患儿出现持续5个月以上的慢性腹泻，5例患儿出现持续2-4周的腹绞痛为主要症状。EAEC感染的发病率为7.7例患者入院每100 000名儿童在一般人群年龄小于16岁。解释EAEC感染与急性，水样腹泻，并可能在工业化国家获得。病因不明的幼儿慢性腹泻或腹绞痛也可能由EAEC感染引起。
Background Enteroaggregative Escherichia coli (EAggEC or EAEC) can spread and cause disease in developing countries, but it is not presently known whether it spreads disease in industrialised countries. Therefore, we did a prospective study to assess the incidence and the clinical manifestations of infections due to EAEC in children in Germany.Methods 798 children with diarrhoea, admitted to hospital within a defined geographical area during a 24-month period, were included in the trial, EAEC were cultured from stool specimens, screened by PCR,and identified by colony hybridisation from DNA sequences found on the virulence plasmid, The findings were confirmed by aggregative adherence to HEp-2 cells. Stool samples from 580 children admitted to hospital without diarrhoea were also studied as controls.Findings EAEC were found in the stools of 16 (2%) of 798 children with diarrhoea, but in none of 580 children without diarrhoea, Only four of the EAEC-infected children had travelled to developing countries. Most EAEC infections were acquired in the summer months. infection with EAEC was associated with acute, watery diarrhoea in 12 children, and with chronic diarrhoea of up to 5 months' duration in four, Five children had abdominal-colic that lasted for 2-4 weeks as their main symptom. The incidence of EAEC infection was 7.7 patients admitted to hospital per 100 000 children in the general population aged younger than 16 years.Interpretation EAEC infection is associated with acute, watery diarrhoea and may be acquired in industrialised countries. Chronic diarrhoea or abdominal colic of unknown aetiology in young children may also be caused by EAEC infection.