Resistance-breaking population of Meloidogyne incognita utilizes plant peroxidase to scavenge reactive oxygen species, thereby promoting parasitism on tomato carrying Mi-1 gene
Resistance-breaking population of Meloidogyne incognita utilizes plant peroxidase to scavenge reactive oxygen species, thereby promoting parasitism on tomato carrying Mi-1 gene
复制标题
南方根结线虫抗性破坏种群利用植物过氧化物酶清除活性氧，从而促进寄生在携带 Mi-1 基因的番茄上
DOI：
10.1016/j.bbrc.2016.11.040
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发表时间：
2017-01-01
影响因子：
3.1
通讯作者：
Li, Hongmei
中科院分区：
文献类型：
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作者：
Guan, Tinglong;Shen, Jinhua;Li, Hongmei
由来自秘鲁茄的Mi-1基因赋予的抗性是有效的并且广泛用于控制根结线虫（RKN，Meloidogyne spp.）。然而，RKN对抗性的破坏严重威胁着抗性资源的持久应用。在这里，一个耐药性破坏种群的M。通过对携带Mi-1的番茄进行连续接种，从无毒群体中筛选出一株南方根结线虫。组织学观察表明，抗性解除群体感染Mi-1番茄后不会引起过敏反应，而无毒群体则会引起过敏反应。利用RNA-seq技术从携带Mi-1的番茄中筛选出308个差异表达基因（DEG）。通过实时定量PCR（qRT-PCR）验证23个选择的DEG的表达模式。随后，烟草脆裂病毒（TRV）介导的RNAi成功地敲除了Mi-1携带番茄中9个高度上调的DEG中的7个。与TRV：：gfp株系相比，以过氧化物酶基因为靶基因的TRV株系表现出更高的活性氧（ROS）水平，并且在抗性破坏群体感染后致病性明显降低。这些结果表明，抗性解除群体可能利用了植物过氧化物酶。Incognita的抗性，从而克服Mi-1介导的抗性。(C)2016 Elsevier Inc. All rights reserved.
Resistance conferred by the Mi-1 gene from Solanum peruvianum is effective and widely used for controlling root-knot nematodes (RKNs, Meloidogyne spp.). However, breakdown of resistance by RKNs seriously threatens the durable application of the resistance resource. Here, a resistance-breaking population of M. incognita was selected from an avirulent population by continuously inoculating on Mi-1carrying tomato. Histological observations showed the resistance-breaking population would not induce hypersensitive response (HR) when infecting Mi-1-carrying tomato, while avirulent population did. A total of 308 differentially expressed genes (DEGs) were identified from Mi-1-carrying tomato upon infection with resistance-breaking versus avirulent populations by RNA-seq. The expression patterns of 23 selected DEGs were validated by quantitative real-time PCR (qRT-PCR). Subsequently, seven out of nine highly up-regulated DEGs were successfully knocked down in Mi-1-carrying tomato by tobacco rattle virus (TRV) mediated RNAi. The TRV line targeting a peroxidase gene showed a much higher magnitude of reactive oxygen species (ROS) and distinct reduction of pathogenicity upon infection of the resistance-breaking population compared with that of TRV::gfp line. Our results suggested that plant peroxidase might be exploited by resistance-breaking population of M. incognita to scavenge ROS, so as to overcome Mi-1-mediated resistance. (C) 2016 Elsevier Inc. All rights reserved.