A CDNA-ENCODING TYROSINASE-RELATED PROTEIN MAPS TO THE BROWN LOCUS IN MOUSE
A CDNA-ENCODING TYROSINASE-RELATED PROTEIN MAPS TO THE BROWN LOCUS IN MOUSE
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DOI：
10.1073/pnas.85.12.4392
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发表时间：
1988-06-01
影响因子：
11.1
通讯作者：
JACKSON, IJ
中科院分区：
文献类型：
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作者：
JACKSON, IJ
小鼠黑色素瘤cDNA克隆是通过其与两种抗酪氨酸酶抗血清（EC 1.14.18.1）的反应性分离的，所述抗酪氨酸酶抗血清来自两个物种，仓鼠和小鼠。cDNA（5A）与另一个pMT 4交叉杂交[Shibahara，S.，Tomita，V.，Sakakura，T.，纳格尔角，巴巴托什角和Muller，R.（1986）Nucleic Acids Res. 14，2413-2427]，先前认为其编码小鼠酪氨酸酶。最近报道了另外两种cDNA，一种人和一种小鼠[Kwon，B.美国，Haq，A. K.，Pomerantz，S. H.和Halaban，R.等人（1987）Proc. Acad. Sci. USA 84，7473-7477;和Yamamoto，H.，Takeuchi，S.，Kudo，T.，Makino，K.，Nakata，A.，Shinoda，T.和Takeuchi，T.（1987）Jpn. J. Genet. 62，271-277]作为酪氨酸酶的候选物，并且它们定位在或非常接近小鼠白化病（c）基因座。它们编码的蛋白质彼此非常相似，但与pMT 4编码的蛋白质不同（尽管相关）。在这里，我使用重组近交系定位pMT 4或接近小鼠棕色（B）基因座。我认为，基因映射到c是真正的酪氨酸酶基因，而映射到B编码酪氨酸酶相关蛋白。实验室菌株中的所有B突变均与相同的诊断性Taq I片段相关，表明所有突变均源自相同的原始突变。我讨论了酪氨酸酶相关蛋白的可能功能。
A mouse melanoma cDNA clone was isolated by virtue of its reactivity with two antisera raised against tyrosinase (EC 1.14.18.1) from two species, hamster and mouse. The cDNA (5A) cross-hybridizes with another, pMT4 [Shibahara, S., Tomita, V., Sakakura, T., Nager, C., Bhabatosh, C. and Muller, R. (1986) Nucleic Acids Res. 14, 2413-2427], previously thought to encode mouse tyrosinase. Two other cDNAs, one human and one mouse, have been reported recently [Kwon, B. S., Haq, A. K., Pomerantz, S. H. and Halaban, R. (1987) Proc. Natl. Acad. Sci. USA 84, 7473-7477; and Yamamoto, H., Takeuchi, S., Kudo, T., Makino, K., Nakata, A., Shinoda, T. and Takeuchi, T. (1987) Jpn. J. Genet. 62, 271-277] as candidates for tyrosinase, and they map at or very close to the mouse albino (c) locus. The proteins they encode are very similar to each other but are distinct from (although related to) the pMT4-encoded protein. Here I use recombinant inbred strains to localize pMT4 at or close to the mouse brown (b) locus. I suggest that the gene mapping to c is the authentic tyrosinase gene, whereas that mapping to b encodes a tyrosinase-related protein. All b mutations in laboratory strains are associated with the same diagnostic Taq I fragment, suggesting that all derive from the same original mutation. I discuss possible function(s) of the tyrosinase-related protein.