EFFECTS OF VITAMIN-D3 METABOLITES ON CALCIUM FLUXES IN INTACT CHICKEN SKELETAL-MUSCLE AND MYOBLASTS CULTURED INVITRO
EFFECTS OF VITAMIN-D3 METABOLITES ON CALCIUM FLUXES IN INTACT CHICKEN SKELETAL-MUSCLE AND MYOBLASTS CULTURED INVITRO
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DOI：
10.1007/bf02405318
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发表时间：
1984-01-01
影响因子：
4.2
通讯作者：
BOLAND, RL
中科院分区：
文献类型：
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作者：
GIULIANI, DL;BOLAND, RL
25-生理浓度的羟基胆钙化醇（250 HD_3）和1，25-二羟基胆钙化醇（1，25 [OH]_2D_3）对培养的维生素D缺乏的鸡比目鱼肌和成肌细胞的Ca ~（2+）流有直接影响。用45 Ca预标记的比目鱼肌的同位素去饱和曲线表明，25 OHD 3的作用定位于缓慢交换的Ca池，在那里它刺激净Ca摄取。1，25（OH）2D 3的主要作用是增加该池的Ca流出速率常数和增加快速交换池的净Ca吸收。24，25-二羟基胆钙化醛对Ca的摄取和流出无活性。1，25（OH）_2D_3能显著增加鸡成肌细胞~（45）Ca标记。这些影响伴随着培养物的生长和分化的变化。显然，25 OHD 3和1，25（OH）2D 3在体内直接参与肌肉细胞Ca。
25-Hydroxycholecalciferol (250HD3) and 1,25-dihydroxycholecalciferol (1,25[OH]2D3) at physiological concentrations exerted direct effects on Ca fluxes in cultured vitamin D-deficient chick soleus muscle and myoblasts. Isotopic desaturation curves of soleus muscle prelabeled with 45Ca indicated that the action of 25OHD3 is localized in a slow-exchangeable Ca pool where it stimulates net Ca uptake. Predominant effects of 1,25(OH)2D3 are an increase of the rate constant of Ca efflux of this pool and in an increase of net Ca uptake in a fast-exchangeable pool. 24,25-Dihydroxycholecalciferal was inactive on Ca uptake and efflux. 1,25(OH)2D3 significantly increased 45Ca labeling of cultured chick myoblasts. These effects were accompanied by changes in the growth and differentiation of the cultures. Apparently, there is a direct involvement in vivo of 25OHD3 and 1,25(OH)2D3 on muscle cellular Ca.