Characterization of ouabain resistant, hypoxanthine phosphoribosyl transferase deficient human cells and their usefulness as a general method for the production of human cell hybrids.
Characterization of ouabain resistant, hypoxanthine phosphoribosyl transferase deficient human cells and their usefulness as a general method for the production of human cell hybrids.
复制标题
哇巴因抗性、次黄嘌呤磷酸核糖基转移酶缺陷人类细胞的表征及其作为生产人类细胞杂交体的通用方法的用途。
DOI：
10.1159/000131536
复制
发表时间：
1980
期刊：
影响因子：
--
通讯作者：
Stanbridge,EJ
中科院分区：
文献类型：
--
作者：
Weissman,B;Stanbridge,EJ
对次黄嘌呤磷酸核糖转移酶缺陷型HeLa细胞和整倍体人成纤维细胞的哇巴因抗性突变体进行了分离和鉴定。这些双突变体被用来检验他们将成为种内人类细胞杂交生产的“通用杂交者”的预测。杂交种是从与不同体细胞来源的人类细胞融合中挑选出来的。此外，还表明，通过对选择系统的改进，可以产生多亲本杂交种，这一结果将对涉及人类细胞的基因剂量实验有用。
Ouabain resistant mutants of hypoxanthine phosphoribosyl transferase deficient HeLa cells and euploid human fibroblasts were isolated and extensively characterized. These double mutants were used to test the prediction that they would be “universal hybridizers” for intraspecific human cell hybrid production. Hybrids were selected from fusions with human cells of diverse somatic origin. In addition it was shown that multi-parental hybrids can be generated using a modification of the selection system, a result which will be useful for gene dosage experiments involving human cells.