MOVEMENT CHARACTERISTICS OF HAMSTER SPERMATOZOA WITHIN THE OVIDUCT
MOVEMENT CHARACTERISTICS OF HAMSTER SPERMATOZOA WITHIN THE OVIDUCT
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DOI：
10.1095/biolreprod22.4.759
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发表时间：
1980-01-01
影响因子：
3.6
通讯作者：
YANAGIMACHI, R
中科院分区：
文献类型：
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作者：
KATZ, DF;YANAGIMACHI, R
采用高速显微摄影技术研究了金仓鼠精子在受精时间和受精部位在输卵管内的运动特征。准备工作包括将切除的输卵管立即浸入矿物油中，并置于37度的深玻片覆盖系统中。C.所有可见的精子都是可运动的，并且表现出鞭毛运动，类似于之前在体外观察中发现的“激活”运动。然而，这种运动的几何细节并不是随着时间的推移而不变的，在更深入的游泳过程中，鞭毛会像鞭子一样弯曲。输卵管上皮表面的存在明显影响了精子的运动轨迹，提示精子与上皮表面的相互作用可能在输卵管精子运输中起作用。
Movement characteristics of golden hamster spermatozoa within the oviduct at the time and site of fertilization were studiedusing high-speed cinemicrography. Preparations consisted of excised oviducts immediately submersed in mineral oil and placed in a deep slide-coverglass system at 37.degree. C. All spermatozoa visualized were motile and were exhibiting flagellar movement similar to the ''activated'' motility previously identified by observations in vitro. The geometrical details of this movement were not constant with time, however, and involved episodes of whiplash-like flagellar bending punctuated by periods of more progressive swimming. The trajectories of the spermatozoa were obviously influenced by the presence of the epithelial surfaces of the oviduct, suggesting that interactions between the spermatozoa and these surfaces could play a role in oviductal sperm transport.