CULTURE OF HUMAN VASCULAR ENDOTHELIAL-CELLS ON A POSITIVELY CHARGED POLYSTYRENE SURFACE, PRIMARIA - COMPARISON WITH FIBRONECTIN-COATED TISSUE-CULTURE GRADE POLYSTYRENE
CULTURE OF HUMAN VASCULAR ENDOTHELIAL-CELLS ON A POSITIVELY CHARGED POLYSTYRENE SURFACE, PRIMARIA - COMPARISON WITH FIBRONECTIN-COATED TISSUE-CULTURE GRADE POLYSTYRENE
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DOI：
10.1016/0142-9612(89)90036-7
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发表时间：
1989-03-01
期刊：
影响因子：
14
通讯作者：
CAZENAVE, JP
中科院分区：
文献类型：
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作者：
KLEINSOYER, C;HEMMENDINGER, S;CAZENAVE, JP
比较了两种培养表面，纤连蛋白包被的组织培养级聚苯乙烯和称为Primaria（Falcon）的表面改性聚苯乙烯。研究了人内皮细胞的形态学（接触抑制和鹅卵石方面）、生物学（血管性血友病因子和前列环素的产生）和生理学（生长活性、非血栓形成性和机械损伤后的再生）特性。低密度和克隆密度的内皮细胞在两种基质上的粘附和生长是相同的，并且生物学特性得到保留。由于细胞外基质在损伤过程中受到破坏，因此在聚苯乙烯上不易研究损伤内皮的再生。尽管如此，Primaria聚苯乙烯可以很容易地取代纤连蛋白涂层在生长实验中，特别是在非常低的接种密度。
Two culture surfaces, fibronectin-coated tissue culture grade polystyrene and a surface-modified polystyrene called Primaria (Falcon), were compared. The morphological (contact inhibition and cobblestone aspect), biological (production of von Willebrand factor and prostacyclin) and physiological (growth activity, non-thrombogenicity and regeneration after mechanical injury) properties of human endothelial cells were studied. Adhesion and growth of endothelial cells at low and clonal density were identical on both substrates and the biological properties were preserved. Regeneration of injured endothelium was less easy to study on Primaria polystyrene because the extracellular matrix was damaged during the lesion process. Nevertheless, Primaria polystyrene can easily be substituted for fibronectin coating in growth experiments, especially at very low seeding density.