Identifying enzyme activities within human saliva which are relevant to dental resin composite biodegradation
Identifying enzyme activities within human saliva which are relevant to dental resin composite biodegradation
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DOI：
10.1016/j.biomaterials.2004.11.001
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发表时间：
2005-07-01
期刊：
影响因子：
14
通讯作者：
Santerre, JP
中科院分区：
文献类型：
--
作者：
Lin, BA;Jaffer, F;Santerre, JP
在全人唾液中检测到与胆固醇和假胆碱酯酶（分别为CE和PCE）相似的酯酶活性。由于商业CE已被证明相对于PCE对牙科复合材料中特定组分具有不同的活性，因此假设从人类唾液中分离的酯酶也将对复合材料中的特定单体元素表现出选择性。本工作的目的是从整个人唾液中分离这些活性，并研究它们对树脂单体，如双苯基缩水甘油酯二甲基丙烯酸酯（芳香结构）和三甘醇二甲基丙烯酸酯（亲水结构），以及对含有后一种单体的固化复合材料的影响。人唾液样品经过处理，在凝胶过滤柱上分离，并测定CE和pc样活性。选择的馏分与上述单体和选择的商业复合材料在37℃下孵育。采用高效液相色谱法监测降解情况。与胆固醇酯酶活性较低但伪胆碱酯酶活性较高的组分相比，具有最高胆固醇酯酶样特征的组分优先降解芳香单体，并显著降解更多的复合材料。因此，唾液腺酯酶对不同的复合树脂组分有偏好。(C) 2004 Elsevier Ltd.版权所有。
Esterase activities similar to those of cholesterol and pseudocholine esterases (CE and PCE, respectively) have been detected within whole human saliva. Since commercial CE has been shown to possess distinct activity relative to PCE for select components in dental composites, it is hypothesized that esterases isolated from human saliva will also show selectivity towards specific monomer elements within the composites. The objective of this work was to carry out the isolation of these activities from whole human saliva and study their individual effects on resin monomers such as Bis-phenyl glycidyl dimethacrylate (aromatic structure) and triethylene glycol dimethacrylate (hydrophilic structure), and on cured composites containing the latter monomers. Human saliva samples were processed, fractionated on a gel filtration column and assayed for CE and PCE-like activity. Selected fractions were incubated at 37 degreesC with the above monomers and select commercial composites. Degradation was monitored using high-performance liquid chromatography. The fraction with the highest cholesterol esterase-like character preferentially degraded the aromatic monomer and significantly degraded more of the composite's material relative to a fraction containing low amounts of the cholesterol esterase activity but elevated pseudocholine esterase-like activity. Hence, it was concluded that select salivary esterases had preferences for distinct composite resin components. (C) 2004 Elsevier Ltd. All rights reserved.