Gallyas- and tau-positive glial structures in motor neuron disease with dementia.
Gallyas- and tau-positive glial structures in motor neuron disease with dementia.
复制标题
运动神经元疾病伴痴呆的 Gallyas 和 tau 阳性神经胶质结构。
作者：
K. Noda;S. Katayama;C. Watanabe;Y. Yamamura;S. Nakamura
我们研究了三例运动神经元病伴痴呆 (MND-D) 尸检病例中的 Gallyas 和 tau 阳性神经胶质结构。 Gallyas 阳性、tau 免疫反应性线状结构和神经胶质细胞中的新月/卷曲内含物主要在海马、海马旁回和杏仁核中观察到。使用 Gallyas 染色和碳酸酐酶 2 (CA2) 免疫组织化学的双重染色显示，少突胶质细胞 CA2 免疫阳性细胞质中出现了一些新月形/卷曲内含物。采用 Gallyas-Braak 方法进行的电子显微镜研究表明，内含物是一种网状、部分致密的物质，在圆形或椭圆形核周围含有 15 nm 的原纤维。由于这些结构出现的区域表现出神经胶质细胞丢失，因此这些数据表明，神经胶质细胞中涉及 tau 蛋白的细胞骨架异常可能与 MND-D 的退行性过程有关。
We have studied Gallyas- and tau-positive glial structures in three autopsied cases of motor neuron disease with dementia (MND-D). Gallyas-positive, tau-immunoreactive thread-like structures in the neuropil and crescent/coiled inclusions in the glial cells were mainly observed in the hippocampus, parahippocampal gyrus, and amygdaloid nucleus. Double staining using Gallyas staining and carbonic anhydrase 2 (CA2) immunohistochemistry revealed that some crescent/coiled inclusions occurred in the CA2-immunopositive cytoplasm of the oligodendroglia. Electron microscopic study with the Gallyas-Braak method revealed that the inclusion was a reticular, partly compact mass, containing 15 nm fibrils around round or oval nuclei. Since the regions where these structures appeared exhibited neuronal loss with gliosis, these data suggest that a cytoskeletal abnormality involving tau protein in glia might be associated with the degenerative process of MND-D.