Cigarette smoke inhibits oocyte cumulus complex pick-up by the oviduct in vitro independent of ciliary beat frequency
Cigarette smoke inhibits oocyte cumulus complex pick-up by the oviduct in vitro independent of ciliary beat frequency
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DOI：
10.1016/s0890-6238(97)00100-7
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发表时间：
1998-01-01
影响因子：
3.3
通讯作者：
Talbot, P
中科院分区：
文献类型：
--
作者：
Knoll, M;Talbot, P
本研究的目的是使用一种新开发的体外试验定量急性暴露于主流（MS）和侧流（SS）烟雾溶液对仓鼠输卵管外植体中卵母细胞卵丘复合体拾取率的影响。实验在手工灌注室中进行，使用来自仓鼠输卵管的漏斗和从成熟卵泡收获的卵母细胞卵丘复合体。通过将染色的卵母细胞卵丘复合物放置在漏斗底部并记录复合物穿过限定路径到达口所需的时间长度来测量卵母细胞卵丘复合物拾取率。向灌注室中加入全MS或SS烟雾溶液导致卵母细胞卵丘复合体拾取率的剂量依赖性降低。出乎意料的是，在用对照培养基冲洗烟雾溶液后，卵母细胞卵丘复合体拾取率继续下降。MS烟雾的气相比颗粒相更具有抑制作用，而SS气相和颗粒相在同等剂量下抑制卵母细胞卵丘复合体拾取率。使用相同的漏斗外植体测量纤毛搏动频率和卵母细胞卵丘复合体拾取速率，以确定烟溶液是否通过抑制纤毛搏动频率来降低卵母细胞卵丘复合体拾取速率，纤毛搏动频率在MS烟溶液中降低，并且在冲洗烟溶液后部分或完全恢复。SS烟雾溶液对纤毛搏动频率无影响或刺激纤毛搏动频率，卵母细胞卵丘复合体拾取率在MS和SS烟雾溶液中均降低，当纤毛搏动频率等于或高于对照时，这些数据表明，卵母细胞卵丘复合体拾取率和纤毛搏动频率可以解偶联，烟雾溶液抑制卵母细胞卵丘复合体拾取。除纤毛搏动频率外，还通过影响卵母细胞卵丘复合体拾取率的因素，对烟雾引起卵母细胞卵丘复合体拾取率下降的可能原因进行了讨论。这些结果可以解释吸烟妇女输卵管不孕和异位妊娠的发病率增加。(C)1998年爱思唯尔科学公司
The purpose of this study was to quantify the effects of acute exposure to mainstream (MS) and sidestream (SS) smoke solutions on oocyte cumulus complex pick-up rate in explants of hamster oviducts using a newly developed in vitro assay, Experiments were performed in handmade perfusion chambers using infundibula from hamster oviducts and oocyte cumulus complexes harvested from mature ovarian follicles. Oocyte cumulus complex pick-up rate was measured by placing a stained oocyte cumulus complex at the base of the infundibulum and recording the length of time needed for the complex to traverse a defined path to the ostium. Addition of either whole MS or SS smoke solutions to the perfusion chamber caused a dose dependent decrease in oocyte cumulus complex pick-up rate, Unexpectedly, upon washout of smoke solutions with control medium, oocyte cumulus complex pick-up rate continued to decline. The gas phase of MS smoke is more inhibitory than the particulate phase, while SS gas and particulate phases inhibit oocyte cumulus complex pick-up rate at equivalent doses. Ciliary beat frequency and oocyte cumulus complex pick-up rate were measured using the same infundibular explants to determine if smoke solutions decrease oocyte cumulus complex pick-up rate by inhibiting ciliary beat frequency, Ciliary beat frequency decreased in MS smoke solutions and recovered either partially or completely after washout of the smoke solutions. SS smoke solutions either produced no change in ciliary beat frequency or stimulated ciliary beat frequency, Oocyte cumulus complex pick-up rate decreased in both MS and SS smoke solutions and further declined during washout when ciliary beat frequency was equivalent to or higher than controls, These data show that oocyte cumulus complex pick-up rate and ciliary beat frequency can be uncoupled and that smoke solutions inhibit oocyte cumulus complex pick-up rate by affecting factors in addition to ciliary beat frequency, Possible reasons for the smoke induced decrease in oocyte cumulus complex pick-up rate are discussed. These results may explain the increased incidence of tubal infertility and ectopic pregnancy observed in women who smoke. (C) 1998 Elsevier Science Inc.