Mouse mammary tumor virus DNA sequences in tumorigenic and nontumorigenic cells from a mammary adenocarcinoma.
Mouse mammary tumor virus DNA sequences in tumorigenic and nontumorigenic cells from a mammary adenocarcinoma.
复制标题
乳腺癌致瘤和非致瘤细胞中的小鼠乳腺肿瘤病毒 DNA 序列。
DOI：
10.1016/0042-6822(82)90325-7
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发表时间：
1982
期刊：
影响因子：
3.7
通讯作者：
McGrath,CM
中科院分区：
文献类型：
--
作者：
Morris,VL;Gray,DA;Jones,RF;Chan,EC;McGrath,CM
如果BALB/c小鼠在C311小鼠上寄养，C311小鼠含有与高乳腺肿瘤发病率相关的乳传播小鼠乳腺肿瘤病毒（MMTV），12个月时其肿瘤发病率从<1 °升至89.5°;此外，这些乳腺肿瘤中整合的MMTV DNA序列增加。为了研究是否所有或只有少数这些添加的MMTV DNA序列与致瘤性，我们分离致瘤性和非致瘤性上皮细胞从一个单一的乳腺癌使用原代甲基纤维素文化。然后使用限制性内切酶分析来检查来自这些培养物的MMTV DNA序列。我们的研究结果表明，甲基纤维素菌落从一个单一的乳腺癌是异质性的，就其集成MMTV DNA序列。我们还发现，并非所有新获得的MMTV DNA拷贝与致瘤性相关。此外，在一个致瘤亚系中，我们仅检测到一个整合拷贝。因此，我们的数据表明，整合的网站是更重要的比整合MMTV DNA序列的总数。此外，致瘤性似乎与MMTV基因组的主要部分的高水平持续转录无关。
If BALB/c mice are foster nursed on C311 mice, which contain milk-borne mouse mammary tumor virus (MMTV) associated with a high mammary tumor incidence, their tumor incidence rises from <1 to 89.5° by 12 months; in addition, there is an increase in the integrated MMTV DNA sequences in these mammary tumors. To investigate if all or only a few of these added MMTV DNA sequences are associated with tumorigenicity, we separated tumorigenic and nontumorigenic epithelial cells from a single mammary adenocarcinoma using primary methocel cultures. The MMTV DNA sequences from these cultures were then examined using restriction endonuclease analysis. Our results indicated that the methocel colonies isolated from a single mammary adenocarcinoma were heterogeneous with respect to their integrated MMTV DNA sequences. We also found that not all newly acquired MMTV DNA copies were associated with tumorigenicity. In addition, in one tumorigenic subline we detected only a single integrated copy. Our data thus suggest that the site of integration is more important than the total number of integrated MMTV DNA sequences. Furthermore, tumorigenicity did not seem to be linked to continued transcription of high levels of a major portion of the MMTV genome.