Yellow-fruited phenotype is caused by 573 bp insertion at 5’ UTR of YFT1 allele in yft1 mutant tomato
Yellow-fruited phenotype is caused by 573 bp insertion at 5’ UTR of YFT1 allele in yft1 mutant tomato
复制标题
黄果表型是由 yft1 突变番茄中 YFT1 等位基因 5 端 UTR 处插入 573 bp 引起的
DOI：
10.1016/j.plantsci.2020.110637
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发表时间：
2020
期刊：
影响因子：
5.2
通讯作者：
Lingxia Zhao
中科院分区：
文献类型：
--
作者：
Weihua Zhao;Lei Gao;Yuhang Li;Minghui Wang;Lida Zhang;Lingxia Zhao
番茄突变体yft 1具有由YFT 1等位基因控制的黄果表型，图位克隆表明该突变体是乙烯不敏感基因2（EIN 2）的同源基因。YFT 1等位基因的遗传损伤归因于转录起始位点下游1,200 bp处的573 bp DNA片段（IF 573）插入。转录组学分析显示，YFT 1损伤导致5，053个差异表达基因。与野生型栽培品种M82相比，yft 1果皮中的DEG。它们被注释为参与乙烯合成、质体发育和类胡萝卜素合成。YFT 1损伤导致其自身转录水平降低，乙烯释放和信号转导受损，色素体发育延迟，类胡萝卜素积累减少。在RNA和DNA水平上研究了下调yft 1中. YFT 1等位基因的分子机制。IF573事件似乎在YFT 1等位基因启动子中引入了位于-272至-173 bp和-172至-73 bp的两个负调控序列，由于引入了异常剪接位点而引起可变剪接，并破坏了上游开放阅读框。在5'-UTR中的一个或多个（uORF）结构。这些结果为番茄果实颜色的分子调控提供了新的思路.
The yft1 tomato mutant has a yellow-fruited phenotype controlled by a recessive gene of YFT1 allele, which has.been shown by map-based cloning to be a homolog of ETHYLENE INSENSITIVE 2 (EIN2). Genetic lesion of YFT1.allele in yft1 is attributed to a 573 bp DNA fragment (IF573) insertion at 1,200 bp downstream of the transcription.start site. Transcriptomic analysis revealed that YFT1 lesion resulted in 5,053 differentially expressed genes.(DEGs) in yft1 pericarp compared with the M82 wild type cultivar. These were annotated as being involved in.ethylene synthesis, chromoplast development, and carotenoid synthesis. The YFT1 lesion caused a reduction in.its own transcript levels in yft1 and impaired ethylene emission and signal transduction, delayed chromoplast.development and decreased carotenoid accumulation. The molecular mechanism underlying the downregulated.YFT1 allele in yft1 was examined at both RNA and DNA levels. The IF573 event appeared to introduce two.negative regulatory sequences located at -272 to -173 bp and -172 to -73 bp in the YFT1 allele promoter, causing.alterative splicing due to introduction of aberrant splicing sites, and breaking upstream open reading frames.(uORF) structure in the 5’-UTR. Those results a new provided insight into molecular regulation of color formation.in tomato fruit.