Thirty complete Streptomyces genome sequences for mining novel secondary metabolite biosynthetic gene clusters
Thirty complete Streptomyces genome sequences for mining novel secondary metabolite biosynthetic gene clusters
复制标题
DOI：
10.1038/s41597-020-0395-9
复制
发表时间：
2020-02-13
期刊：
影响因子：
9.8
通讯作者：
Cho, Byung-Kwan
中科院分区：
文献类型：
--
作者：
Lee, Namil;Kim, Woori;Cho, Byung-Kwan
链霉菌是具有重要工业重要性的革兰氏阳性细菌，因为它们能够产生多种抗生素和生物活性次级代谢产物。基因组挖掘的最新进展表明，链霉菌基因组拥有大量未经探索的沉默次生代谢物生物合成基因簇（smBGC）。这表明链霉菌基因组仍然是新药发现的宝贵来源。在这里，我们展示了 22 种链霉菌属物种和 8 种不同的委内瑞拉链霉菌菌株的高质量基因组序列，这些序列是通过利用长读长和短读长基因组测序方法的混合策略组装而成的。组装的基因组基因空间完整性超过97.4%，总长度范围为6.7至10.1 Mbp。他们的注释平均识别出 7,000 个蛋白质编码基因、20 个 rRNA 和 68 个 tRNA。 smBGC 的计算机预测总共识别出 922 个簇，其中包括许多产物未知的簇。我们预计这些基因组的可用性将加速链霉菌中新型次级代谢产物的发现，并阐明复杂的 smBGC 调控。
Streptomyces are Gram-positive bacteria of significant industrial importance due to their ability to produce a wide range of antibiotics and bioactive secondary metabolites. Recent advances in genome mining have revealed that Streptomyces genomes possess a large number of unexplored silent secondary metabolite biosynthetic gene clusters (smBGCs). This indicates that Streptomyces genomes continue to be an invaluable source for new drug discovery. Here, we present high-quality genome sequences of 22 Streptomyces species and eight different Streptomyces venezuelae strains assembled by a hybrid strategy exploiting both long-read and short-read genome sequencing methods. The assembled genomes have more than 97.4% gene space completeness and total lengths ranging from 6.7 to 10.1 Mbp. Their annotation identified 7,000 protein coding genes, 20 rRNAs, and 68 tRNAs on average. In silico prediction of smBGCs identified a total of 922 clusters, including many clusters whose products are unknown. We anticipate that the availability of these genomes will accelerate discovery of novel secondary metabolites from Streptomyces and elucidate complex smBGC regulation.