Bioengineering and application of novel glucose polymers
Bioengineering and application of novel glucose polymers
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DOI：
10.1080/1024240310001614379
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发表时间：
2003-08-01
影响因子：
1.8
通讯作者：
Kuriki, T
中科院分区：
文献类型：
--
作者：
Fujii, K;Takata, H;Kuriki, T
利用葡聚糖磷酸化酶、支化酶和4-α-葡聚糖转移酶制备了分子大小和结构可控的葡萄糖聚合物。直链葡聚糖的相对分子质量由葡萄糖-1-磷酸与底物的摩尔比严格控制，分支酶/葡聚糖磷酸化酶的相对活性比严格控制支链葡聚糖的相对分子质量。4-α-葡聚糖转移酶和支链酶在直链淀粉和支链淀粉上发生环化反应生成环状葡聚糖。环状葡聚糖的大小和结构受所选择的酶和底物的类型以及反应条件的控制。这种体外方法可用于制备具有应用价值的新型葡萄糖聚合物。
Glucan phosphorylase, branching enzyme, and 4-alpha-glucanotransferase were employed to produce glucose polymers with controlled molecular size and structures. Linear or branched glucan was produced from glucose-1-phosphate by glucan phosphorylase alone or together with branching enzyme, where the molecular weight of linear glucan was strictly controlled by the glucose-1-phosphate/primer molar ratio, and the branching pattern by the relative branching enzyme/glucan phosphorylase activity ratio. Cyclic glucans were produced by the cyclization reaction of 4-alpha-glucanotransferases and branching enzyme on amylose and amylopectin. Molecular size and structure of cyclic glucan was controlled by the type of enzyme and substrate chosen and by the reaction conditions. This in vitro approach can be used to manufacture novel glucose polymers with applicable value.