Effects of Epicatechin on Rat Islets of Langerhans
Effects of Epicatechin on Rat Islets of Langerhans
复制标题
表儿茶素对大鼠胰岛的影响
DOI：
10.2337/diab.33.3.291
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发表时间：
1984
期刊：
影响因子：
7.7
通讯作者：
S. Howell
中科院分区：
文献类型：
--
作者：
C. Hii;S. Howell
在印度医学中，含有表儿茶素（一种类黄酮）的植物已被用于治疗糖尿病。本研究报告了该化合物对离体胰岛的影响。发现类黄酮（1 mM）在2或20 mM葡萄糖存在下，在静态孵育或灌流中增加分离的大鼠胰岛的胰岛素分泌。表儿茶素介导的胰岛素分泌的增加是ATP和温度依赖性的。超微结构研究表明，在暴露于化合物5天后，B细胞的结构没有有害变化。每天两次腹腔注射30 mg/kg体重的表儿茶素，连续4天，使胰岛胰岛素含量增加30%。与注射水的对照组相比，当暴露于20 mM葡萄糖时，从注射表儿茶素的大鼠分离的胰岛中分泌的胰岛素显著增加。此外，用5.5 mM葡萄糖培养4天的成年大鼠胰岛在0.05 mM表儿茶素的存在下显示出DNA合成的显著增加。这些结果表明表儿茶素对胰岛功能的各个方面的直接影响。
Plants containing epicatechin (a flavonoid) have been used to treat diabetes mellitus in Indian medicine. The present study reports effects of this compound on isolated islets of Langerhans. The flavonoid (1 mM) was found to increase insulin secretion from isolated rat islets of Langerhans in the presence of either 2 or 20 mM glucose, in static incubations, or in perifusion. The increase in insulin secretion mediated by epicatechin was both ATP- and temperature-dependent. Ultrastructural studies showed no deleterious changes in the structure of the B-cells after 5 days of exposure to the compound. Intraperitoneal injection of 30 mg/kg body wt of epicatechin twice daily for 4 days increased the islet insulin content by 30%. Secretion of insulin from islets isolated from epicatechin-injected rats was significantly increased when exposed to 20 mM glucose in comparison with water-injected controls. Furthermore, islets of adult rats cultured with 5.5 mM glucose for 4 days showed a significant increase in DNA synthesis in the presence of 0.05 mM epicatechin. These results suggest direct effects of epicatechin on various aspects of islet function.