Simultaneous quantification of three major bioactive triterpene acids in the leaves of Diospyros kaki by high-performance liquid chromatography method
Simultaneous quantification of three major bioactive triterpene acids in the leaves of Diospyros kaki by high-performance liquid chromatography method
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高效液相色谱法同时定量柿叶中三种主要生物活性三萜酸
DOI：
10.1016/j.jpba.2006.01.044
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发表时间：
2006-06-07
影响因子：
3.4
通讯作者：
He, Chao-Hong
中科院分区：
文献类型：
--
作者：
Fan, Jie-Ping;He, Chao-Hong
柿树叶是一种传统中药，用于治疗各种疾病。为提高D.建立了一种简便、快速、准确的同时测定三种具有生物活性的三萜酸：巴宾酸（BA）及其差向异构体、轮桐酸（RA）、沿着和24-羟基熊果酸（HA）的高效液相色谱（HPLC）分析方法。在反相C-18色谱柱上，以甲醇和磷酸水溶液作为移动的相，使用210 nm的监测波长进行HPLC含量测定。该方法已成功地应用于五种不同溶剂提取物中这三种生物活性三萜酸的定量分析。中国六个不同地方的柿树和树叶。结果表明，提取溶剂和提取部位对各主要活性成分的总含量和含量有很大的影响，表明该方法可有效地控制竹叶中活性成分的质量。柿蒂提取物和植物药是确保其临床效益的关键。同时采用低选择性比色法测定总三萜的含量，并与高效液相色谱法进行了比较。(c)2006 Elsevier B. V.保留所有权利。
The leaf of Diospyros kaki, which is a traditional Chinese medicine, has been used for the treatment of various diseases. In order to improve the quality assurance of the leaves of D. kaki, derived extracts and phytomedicines, a simple, rapid and accurate high-performance liquid chromatography (HPLC) method was developed to simultaneously assess the three bioactive triterpene acids: barbinervic acid (BA) and its epimer, rotungenic acid (RA), along with 24-hydroxy ursolic acid (HA). This HPLC assay was performed on a reversed-phase C-18 column with methanol and aqueous H3PO4 as the mobile phase and using a monitoring wavelength at 210 nm. This method was successfully applied to quantify these three bioactive triterpene acids in five different solvent extracts of the leaves of D. kaki and in the leaves from six different locations in China. The results demonstrated the total content and quantity of each of the main bioactive compounds were strongly dependent on the extraction solvents and locations, indicating that the quality control of the bioactive ingredients in the leaves of D. kaki, derived extracts and phytomedicines is critical to ensure its clinical benefits. The content of the total triterpenoids was also determined by the less selective colorimetric method, and the comparison with the HPLC method was given. (c) 2006 Elsevier B.V. All rights reserved.