Proteomic analysis of the flooding tolerance mechanism in mutant soybean
Proteomic analysis of the flooding tolerance mechanism in mutant soybean
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DOI：
10.1016/j.jprot.2012.12.023
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发表时间：
2013-02-21
影响因子：
3.3
通讯作者：
Nishimura, Minoru
中科院分区：
文献类型：
--
作者：
Komatsu, Setsuko;Nanjo, Yohei;Nishimura, Minoru
大豆的淹水胁迫是一个严重的问题，因为它降低了生长；然而，还没有鉴定出耐淹水的品种。为了分析大豆的耐涝机理，利用蛋白质组学技术对大豆耐涝突变体进行了分离和分析。利用伽马射线辐照的大豆进行了5次耐涝试验，其根系生长(M6期)即使在淹水胁迫下也没有受到抑制。对两天大的野生型和突变体植株进行了为期两天的淹水胁迫，并利用基于凝胶的蛋白质组学技术对蛋白质进行了鉴定。在淹水胁迫下，野生型与发育、蛋白质合成/降解、次生代谢和细胞壁相关的蛋白质水平发生了变化，但这些蛋白质在突变体中没有明显差异。相反，在淹水胁迫下，突变株中与发酵相关的蛋白质数量增加。突变体植株的根尖不受淹水胁迫的影响，即使野生型植株的根部受到了伤害。在淹水胁迫初期，突变体的乙醇脱氢酶活性高于野生型。综上所述，这些结果表明，在淹水初期，发酵系统的激活可能是获得大豆耐淹性的一个重要因素。(C)2013爱思唯尔B.V.保留所有权利。
Flooding stress of soybean is a serious problem because it reduces growth; however, flooding-tolerant cultivars have not been identified. To analyze the flooding tolerance mechanism of soybean, the flooding-tolerant mutant was isolated and analyzed using a proteomic technique. Flooding-tolerance tests were repeated five times using gamma-ray irradiated soybeans, whose root growth (M6 stage) was not suppressed even under flooding stress. Two-day-old wild-type and mutant plants were subjected to flooding stress for 2 days, and proteins were identified using a gel-based proteomic technique. In wild-type under flooding stress, levels of proteins related to development, protein synthesis/degradation, secondary metabolism, and the cell wall changed; however, these proteins did not markedly differ in the mutant. In contrast, an increased number of fermentation-related proteins were identified in the mutant under flooding stress. The root tips of mutant plants were not affected by flooding stress, even though the wild-type plants had damaged root. Alcohol dehydrogenase activity in the mutant increased at an early stage of flooding stress compared with that of the wild-type. Taken together, these results suggest that activation of the fermentation system in the early stages of flooding may be an important factor for the acquisition of flooding tolerance in soybean. (C) 2013 Elsevier B.V. All rights reserved.