Locations of three repetitive sequence families found in BALB/c adult beta-globin clones.
Locations of three repetitive sequence families found in BALB/c adult beta-globin clones.
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BALB/c 成人 β-珠蛋白克隆中发现的三个重复序列家族的位置。
DOI：
10.1093/nar/9.5.1133
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发表时间：
1981
影响因子：
14.9
通讯作者：
Edgell,MH
中科院分区：
文献类型：
--
作者：
Haigwood,NL;Jahn,CL;Hutchison3rd,CA;Edgell,MH
在克隆的BALB/c（Hddd）小鼠β-珠蛋白基因的EcoRI片段中，定位了三个不同的重复序列。一个序列“a”位于β-主基因3′端1.5-2 kb处。第二个“B”位于β-微效基因的5′端4kb和3′端7.5kb。含有β-微效基因的14 kbEcoRI片段至少带有一个额外的重复元件“c”。用每个重复序列探测BALB/c DNA文库已经证明，这些序列是中度至高度重复的，并且在整个基因组中彼此广泛散布。此外，重复序列“a”和“B”分别优先存在于卫星和主带DNA中。通过用每个重复序列探测非成体珠蛋白克隆，证明了这些重复序列在β-珠蛋白簇中其他地方的出现。这些重复序列围绕非成体基因的排列方式为5′-“B”-“B”-εy-βh1-βh2-“c”-βh3-3′。用这些重复序列探测C57 BL/10（HbbS）成人基因克隆表明，这两种单倍型的成人区域中这些序列的分布基本相同。
Three different repeat sequences have been mapped within the cloned EcoRI fragments that Contain the adult β-globin genes from the BALB/c (Hddd) mouse. One sequence, “a”, occurs 1.5–2 kb 3′ to the β-major gene. A second, “b”, is found 4kb 5′ and 7.5kb 3′ to the β-minor gene. The 14kb EcoRI fragment bearing the β-minor gene carries at least one additional repetitive element, “c”. Probing a BALB/c DNA library with each repeat has demonstrated that these sequences are moderately to highly repetitive and are extensively interspersed with each other throughout the genome. In addition, repeats “a” and “b” are preferentially found in satellite and main-band DNA, respectively. The occurence of these repeats elsewhere in the β-globin cluster was demonstrated by probing the non-adult globin clones with each repeat. The arrangement of these repeats around the non-adult genes is 5′–“b”–“b”–εy–βh1–βh2–“c”–βh3–3′. Probing the C57BL/10 (HbbS) adult gene clones with these repeats demonstrated that the distribution of these sequences in the adult region of these two haplotypes is essentially the same.