Regeneration from leaf segments of in vitro‐grown shoots of Malus baccata
Regeneration from leaf segments of in vitro‐grown shoots of Malus baccata
复制标题
体外生长的海棠芽叶段的再生
DOI：
10.1080/01140670809510239
复制
发表时间：
2008
影响因子：
1.3
通讯作者：
Zhenhai Han
中科院分区：
文献类型：
--
作者：
Chun;Yi Wang;Xuefeng Xu;Yang Sun;L. Zhu;Zhenhai Han
摘要 利用离体培养的海棠芽的叶外植体研究了不定芽再生。使用补充有 4 毫克/升 6-苄基腺嘌呤和 0.5 毫克/升 α-萘-乙酸的 Murashige 和 Skoog 培养基获得了最佳再生。暗培养10天给出了不定芽再生的最佳结果。山梨醇被证明是最适合M. baccata 芽再生的碳源。在芽再生中，用 Phytagel 或 gelrite 进行凝胶化比琼脂更有效。最高再生频率为97%，每个再生叶外植体的最大芽数为8.7。在大多数情况下，添加抗生素卡那霉素或潮霉素会显着降低再生能力。本研究中再生方案的制定将有助于该物种未来的转化工作。
Abstract Adventitious shoot regeneration was investigated using leaf explants of in vitro cultured shoots of Malus baccata. Optimal regeneration was obtained using Murashige and Skoog medium supplemented with 4 mg/litre 6‐benzyladenine and 0.5 mg/litre α‐naphthalene‐acetic acid. Dark incubation for 10 days gave the best results of adventitious shoot regeneration. Sorbitol was proved to be the most suitable carbon source for shoot regeneration in M. baccata. gelling with either Phytagel or gelrite was more efficient than agar in shoot regeneration. The highest regeneration frequency was 97% and the greatest shoot number per regenerated leaf explant was 8.7. The addition of antibiotics Kanamycin or Hygromycin significantly reduced regeneration capacity in most instances. The development of the regeneration protocol in this study will facilitate the future transformation work in this species.