Interactions between growth cones and neurites growing from different neural tissues in culture
Interactions between growth cones and neurites growing from different neural tissues in culture
复制标题
培养物中不同神经组织生长的生长锥和神经突之间的相互作用
作者：
J. Kapfhammer;J. Raper
我们之前使用视网膜和交感神经外植体来证明生长锥能够识别培养物中的特定神经突并从中缩回（Kapfhammer 等人，1986）。为了确定这种现象的普遍性，并了解它可以检测到多少种不同的神经突标签，我们在体外研究了个体生长锥和从各种神经源延伸的神经突之间的相互作用。利用交感神经元、睫状神经元、背根神经节（DRG）、视网膜神经元和间脑神经元之间的大多数可能配对，我们发现在大多数情况下：（1）生长锥不会从源自同一组织的神经突中缩回； (2) 视网膜生长锥不从间脑神经突回缩； (3) 交感神经、睫状体和背根神经节生长锥，除了一种可能的例外，不会从交感神经、睫状体或背根神经节神经突缩回； (4) 视网膜生长锥从交感神经、睫状体和背根神经节神经突缩回； (5)交感神经、睫状体和背根神经节生长锥从视网膜神经突缩回； (6)交感生长锥从间脑神经突缩回。与这些结果一致的一个简单假设是，存在两种标签——一种与中枢神经突相关，另一种与周围神经突相关——并且外周生长锥被编程为从中央标签缩回，而中央生长锥被编程为从交感神经、睫状体和背根神经节神经突缩回； (5)与发育过程中CNS和PNS分离有关。
We have previously used retinal and sympathetic explants to show that growth cones recognize and retract from specific neurites in culture (Kapfhammer et al., 1986). In an effort to determine the generality of this phenomenon and to see how many different neurite labels can be detected by it, we have studied interactions between individual growth cones and neurites extending from a variety of neural sources in vitro. Using most of the possible pairings between sympathetic, ciliary, dorsal root ganglion (DRG), retinal, and diencephalic neurons, we have found that in most instances: (1) Growth cones do not retract from neurites originating from the same tissue; (2) retinal growth cones do not retract from diencephalic neurites; (3) sympathetic, ciliary, and DRG growth cones, with one possible exception, do not retract from sympathetic, ciliary, or DRG neurites; (4) retinal growth cones retract from sympathetic, ciliary, and DRG neurites; (5) sympathetic, ciliary, and DRG growth cones retract from retinal neurites; and (6) sympathetic growth cones retract from diencephalic neurites. A simple hypothesis consistent with these results is that 2 labels exist--one associated with central neurites and another associated with peripheral neurites-- and that peripheral growth cones are programmed to retract from the central label and central growth cones are programmed to retract from sympathetic, ciliary, and DRG neurites; (5) symevant to the separation of the CNS and PNS during development.