Human placental Na+,K+-ATPase alpha subunit: cDNA cloning, tissue expression, DNA polymorphism, and chromosomal localization.
Human placental Na+,K+-ATPase alpha subunit: cDNA cloning, tissue expression, DNA polymorphism, and chromosomal localization.
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人胎盘 Na ,K -ATP 酶 α 亚基：cDNA 克隆、组织表达、DNA 多态性和染色体定位。
DOI：
10.1073/pnas.84.22.7901
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发表时间：
1987
影响因子：
11.1
通讯作者：
Blostein,R
中科院分区：
文献类型：
--
作者：
Chehab,FF;Kan,YW;Law,ML;Hartz,J;Kao,FT;Blostein,R
一种2.2-ATP酶克隆，其包含Na/sup +/，K/sup +/-ATP酶编码序列的主要部分。阿尔法帽。从人胎盘组织中克隆了一个亚基，其序列与编码人胎盘组织的亚基完全相同。阿尔法帽。人肾和HeLa细胞的亚基。对来自各种人体组织和细胞系的Na/sup +/，K/sup +/-ATP酶基因的mRNA产物的转移印迹分析显示只有一条带（约1.5 μ g/ml）。= 4.7个内切酶）。组织中的表达水平为肠>胎盘>肝>胰腺，细胞系中的表达水平为人红白血病>丁酸盐诱导的结肠>结肠>脑> HeLa细胞。mRNA在网织红细胞中检测不到，这与作者未能在大小选择的（> 2个酶）Adagt 11网织红细胞cDNA文库中检测到阳性克隆一致。DNA分析表明，多态性的EcoRI带和染色体定位的流式分选和原位杂交表明，...阿尔法帽。亚基位于1号染色体的短臂（p11-p13带）上。
A 2.2-kilobase clone comprising a major portion of the coding sequence of the Na/sup +/, K/sup +/-ATPase ..cap alpha.. subunit was cloned from human placenta and its sequence was identical to that encoding the ..cap alpha.. subunit of human kidney and HeLa cells. Transfer blot analysis of the mRNA products of the Na/sup +/, K/sup +/-ATPase gene from various human tissues and cell lines revealed only one band (approx. = 4.7 kilobases) under low and high stringency washing conditions. The levels of expression in the tissues were intestine > placenta > liver > pancreas, and in the cell lines the levels were human erythroleukemia > butyrate-induced colon > colon > brain > HeLa cells. mRNA was undetectable in reticulocytes, consistent with the authors failure to detect positive clones in a size-selected ( > 2 kilobases) lambdagt11 reticulocyte cDNA library. DNA analysis revealed by a polymorphic EcoRI band and chromosome localization by flow sorting and in situ hybridization showed that the ..cap alpha.. subunit is on the short is on the short arm (band p11-p13) of chromosome 1.