Nampt maintains mitochondrial content via Nrf2-PPARα/AMPKα pathway to promote cell survival under oxidative stress
Nampt maintains mitochondrial content via Nrf2-PPARα/AMPKα pathway to promote cell survival under oxidative stress
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Nampt 通过 Nrf2-PPARα/AMPKα 途径维持线粒体含量，以促进氧化应激下的细胞存活
DOI：
10.1016/j.cellsig.2019.109496
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发表时间：
2019
影响因子：
4.8
通讯作者：
Xiaodong Chen
中科院分区：
文献类型：
--
作者：
An Yu;Ronghua Zhou;Benzeng Xia;Weiwei Dang;Zaiqing Yang;Xiaodong Chen
Mitochondria plays a key role in regulating cell death process under stress conditions and it has been indicated that NAMPT overexpression promotes cell survival under genotoxic stress by maintaining mitochondrial NAD+level. NAMPT is a rate-limiting enzyme for NAD+production in mammalian cells and it was suggested that NAMPT and NMNAT3 are responsible for mitochondrial NAD+production to maintain mitochondrial NAD+pool. However, subsequent studies suggested mitochondrial may lack the NAMPT-NMANT3 pathway to maintain NAD+level. Therefore, how NAMPT overexpression rescues mitochondrial NAD+content to promote cell survival in response to genotoxic stress remains elusive. Here, we show that NAMPT promotes cell survival under oxidative stress via both SIRT1 dependent p53-CD38 pathway and SIRT1 independent NRF2-PPARα/AMPKα pathway, and the NRF2-PPARα/AMPKα pathway plays a more profound role in facilitating cell survival than the SIRT1-p53-CD38 pathway does. Mitochondrial content and membrane potential were significantly reduced in response to H2O2 treatment, whereas activated NRF2-PPARα/AMPKα pathway by NAMPT overexpression rescued the mitochondrial membrane potential and content, suggesting that maintained mitochondrial content and integrity by NAMPT overexpression might be one of the key mechanisms to maintain mitochondrial NAD+level and subsequently dictate cell survival under oxidative stress. Our results indicated that NRF2 is a novel down-stream target of NAMPT, which mediates anti-apoptosis function of NAMPT via maintaining mitochondrial content and membrane potential.
