Screening Hepatotoxic Components in Euodia rutaecarpa by UHPLC-QTOF/MS Based on the Spectrum-Toxicity Relationship
Screening Hepatotoxic Components in Euodia rutaecarpa by UHPLC-QTOF/MS Based on the Spectrum-Toxicity Relationship
复制标题
基于谱-毒性关系的UHPLC-QTOF/MS筛选吴茱萸肝毒性成分
DOI：
10.3390/molecules22081264
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发表时间：
2017-08-01
期刊：
影响因子：
4.6
通讯作者：
Yuan, Jinbin
中科院分区：
文献类型：
--
作者：
Liang, Jian;Chen, Yang;Yuan, Jinbin
朱茱萸是临床上常用的中药，具有镇痛止咳的作用；然而，随着越来越多的报告显示它是有毒的，特别是肝毒性，引起人们对其毒性原因的关注也越来越多。本研究采用基于谱效应的分析方法，筛选乌桕中主要的肝毒性成分，阐明其毒性物质的基础。建立了超高效液相色谱-四极杆飞行时间质谱仪（UHPLC-QTOF/MS）指纹图谱方法，对石楠提取物（石油醚、氯仿、乙酸乙酯、正丁醇和水）进行指纹图谱分析。采用正交偏最小二乘法（ops）建立了与小鼠血清中谷丙转氨酶（ALT）和天冬氨酸转氨酶（AST）水平作为肝损伤评价指标的毒谱关系。建立了UHPLC-MS指纹图谱，OPLS分析结果表明，松柏素、1-甲基-2-十一烷基-4(1H)-喹诺酮、1-甲基-2-[(6Z, 9Z, 12E)-戊卡三烯]-4(1H)-喹诺酮、依卡平、1-甲基-2-[(Z)-7-三烯基]-4(1H)-喹诺酮、二氢依卡平、1-甲基-2-十四烷基-4(1H)-喹诺酮可能与乌龙果的肝毒性有关。本文为同类毒性中药毒性物质基础的基础研究提供了相当的方法和见解。
Euodia rutaecarpa is a common traditional Chinese medicine (TCM) in clinical practice, having the ability to suppress pain and cease coughing; however, with the increasing reports showing that it is toxic, particularly hepatotoxic, the concerns raised by what cause its toxicity is growing. In the current study, an analysis method based on the spectrum effect has been employed to screen the major hepatotoxic components in Euodia rutaecarpa so that the toxic material's basis would be elucidated. A fingerprinting method of the Euodia rutaecarpa extracts (which were petroleum ether, chloroform, ethyl acetate, n-butanol, and water) using ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometer (UHPLC-QTOF/MS) has been developed. Orthogonal partial least squares (OPLS) was used to establish the spectrum-toxicity relationship with the levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in mice serum as evaluation indices for liver injury. The UHPLC-MS fingerprint was established and the OPLS analytical results suggested that coniferin, 1-methyl-2-undecyl-4(1H)-quinolone, 1-methyl-2-[(6Z, 9Z, 12E)-pentadeca triene]-4(1H)-quinolone, evocarpine, 1-methyl-2-[(Z)-7-tridecenyl]-4(1H)-quinolone, dihydroevocarpine, and 1-methyl-2-tetradecy-4-(1H)-quinolone probably associated with the hepatotoxicity of Euodia rutaecarpa. This paper offered considerable methods and insight for the fundamental research of the toxic material basis of similar toxic TCMs.