Molecular characterization of two novel transporters from human and mouse kidney and from LLC-PK1 cells reveals a novel conserved family that is homologous to bacterial and Aspergillus nucleobase transporters
Molecular characterization of two novel transporters from human and mouse kidney and from LLC-PK1 cells reveals a novel conserved family that is homologous to bacterial and Aspergillus nucleobase transporters
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DOI：
10.1016/s0167-4781(98)00151-1
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发表时间：
1998-11-08
期刊：
影响因子：
--
通讯作者：
Holtzman, EJ
中科院分区：
文献类型：
--
作者：
Faaland, CA;Race, JE;Holtzman, EJ
碱基转运对于核酸和抗病毒药物的代谢是重要的。这种转运已在几种哺乳动物细胞中进行了功能性描述，但尚未得到很好的分子表征。我们报告了两个新的转运蛋白的克隆。YSPL2编码650个残基的蛋白，并且具有普遍存在的8kb转录本。人类和猪的同源物有95%相似。YSPL3编码一个598个残基的蛋白质，其转录物为3 kb，仅在肾脏和肝脏中表达。人YSPL2和YSPL3在氨基酸水平上有60%的相似性，并且都显示出与脊椎动物中描述的第一个核碱基通透酶基因YSPL1有31%的相似性。这些蛋白质似乎是一个新的可能的核碱基转运蛋白家族的成员，与细菌和曲霉属的核碱基转运蛋白具有显着的序列相似性。需要进一步的功能研究来揭示这些转运蛋白在正常和疾病状态下核酸代谢中的作用。(C)1998 Elsevier Science B.V.保留所有权利。
Nucleobase transport is important for the metabolism of nucleic acids and antiviral and antineoplastic drugs. This transport has been functionally described in several mammalian cells but has not been well characterized molecularly. We report the cloning of two novel transporters. YSPL2 encodes a 650-residue protein and has an ubiquitous 8 kb transcript. The human and pig homologs are 95% similar. YSPL3 encodes a 598-residue protein with a 3 kb transcript that is expressed only in kidney and liver. Human YSPL2 and YSPL3 are 60% similar at the amino acid level and both show 31% similarity to the first nucleobase permease gene described in vertebrates, YSPL1. These proteins appear to be members of a new family of possible nucleobase transporters with significant sequence similarities with bacterial and Aspergillus nucleobase transporters. Further functional studies will be needed to unveil the role of these transporters in nucleic acid metabolism in normal and in disease states. (C) 1998 Elsevier Science B.V. All rights reserved.