Identification of a naturally occurring recombinant genotype 2/6 hepatitis C virus
Identification of a naturally occurring recombinant genotype 2/6 hepatitis C virus
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DOI：
10.1128/jvi.00312-06
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发表时间：
2006-08-01
影响因子：
5.4
通讯作者：
Haagmans, Bart L.
中科院分区：
文献类型：
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作者：
Noppornpanth, Suwanna;Lien, Truong Xuan;Haagmans, Bart L.
丙型肝炎病毒（HCV）显示出高水平的序列多样性，目前分为六种基因型和越来越多的亚型。最有可能的是，这种异质性是由遗传漂变引起的;重组的证据很少。为了研究越南HCV的分子异质性，我们通过CORE和NS 5 B区域的序列分析，分析了58份来自越南献血员的HCV RNA阳性血清。系统发育分析显示存在基因型1（38%），基因型2（10.3%）和基因型6（51.7%）的病毒。除2份（D3和D54）外，所有样品均显示一致结果。样品D54是基因型2 i和6 h病毒的混合感染。另一方面，样品D3的全基因组分析和bootscan分析揭示了在5'和3'端分别具有基因型2 i和基因型6p序列的重组病毒。交叉点位于NS 2/NS 3连接处的核苷酸位置3405至3464（根据原型毒株HCV-H，M67463编号）之间。这种天然存在的重组病毒的鉴定加强了重组可能在HCV流行病学和进化中发挥作用的概念。此外，重组断裂点的位置可能与构建感染性嵌合病毒有关。
Hepatitis C viruses (HCVs) display a high level of sequence diversity and are currently classified into six genotypes and an increasing number of subtypes. Most likely, this heterogeneity is caused by genetic drift; evidence for recombination is scarce. To study the molecular heterogeneity of HCV in Vietnam, we analyzed 58 HCV RNA-positive sera from Vietnamese blood donors by sequence analysis of the CORE and NS5B regions. Phylogenetic analyses revealed the presence of genotype 1 (38%), genotype 2 (10.3%), and genotype 6 viruses (51.7%). All samples showed concordant results except for two (D3 and D54). Sample D54 was a mixed infection of genotype 2i and 6h viruses. Whole-genome analysis and bootscan analysis of sample D3, on the other hand, revealed a recombinant virus with genotype 2i and genotype 6p sequences at the 5' and 3' ends, respectively. The crossover point was located between nucleotide positions 3405 to 3464 (numbering according to prototype strain HCV-H, M67463) at the NS2/NS3 junction. The identification of this naturally occurring recombinant virus strengthens the concept that recombination may play a role in HCV epidemiology and evolution. Furthermore, the location of the recombination breakpoint may be relevant for constructing infectious chimeric viruses.