A carbon column-based liquid chromatography electrochemical approach to routine 8-hydroxy-2′-deoxyguanosine measurements in urine and other biologic matrices:: A one-year evaluation of methods
A carbon column-based liquid chromatography electrochemical approach to routine 8-hydroxy-2′-deoxyguanosine measurements in urine and other biologic matrices:: A one-year evaluation of methods
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DOI：
10.1016/s0891-5849(99)00113-6
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发表时间：
1999-09-01
影响因子：
7.4
通讯作者：
Matson, WR
中科院分区：
文献类型：
--
作者：
Bogdanov, MB;Beal, MF;Matson, WR
8-羟基-2 '-脱氧鸟苷（80 H2'dG）是羟自由基损伤DNA的主要稳定标志物。它与多种疾病和环境损伤有关，并已被提议作为氧化应激的有用系统标记物。DNA印迹中80 H2 'dG的分析方法已经很好地建立;然而，在其他体液如尿液或血浆中游离80 H2'dG的常规测量一直存在问题。这阻碍了其作为一般临床、治疗监测或环境评估工具的评价。因此，我们开发了一种液相色谱电化学柱切换系统，该系统基于使用多孔碳柱的独特嘌呤选择性，其允许在各种生物基质中常规精确测量80 H2 'dG。本文描述了系统设计的基本原理和为尿液、血浆、脑脊液、组织、DNA、唾液、汗液、肾透析液、食物、粪便、培养基质和微透析液中的80 H2 'dG开发的方案。报告了人类和动物体液和组织中的浓度。在对应用于约3600份样本的方法进行1年评价的背景下讨论了系统性能，使用内部质量控制和外部盲法检测来确定长期准确度。这些方法是可靠和准确的，因此应该证明在评估氧化DNA损伤在衰老和人类疾病中的作用和效用方面是有用的。(C)1999 Elsevier Science Inc.
8-Hydroxy-2'-deoxyguanosine (80H2'dG) is a principal stable marker of hydroxyl radical damage to DNA. It has been related to a wide variety of disorders and environmental insults, and has been proposed as a useful systematic marker of oxidative stress. Analytic procedures for 80H2'dG in DNA digests are well established; however, routine measurement of free 8OH2'dG in other body fluids such as urine or plasma has been problematic. This has hindered its evaluation as a general clinical, therapeutic monitoring, or environmental assessment tool. Therefore, we developed a liquid chromatography electrochemical column-switching system based on the use of the unique purine selectivity of porous carbon columns that allows routine accurate measurement of 80H2'dG in a variety of biologic matrices. This paper describes the rationale of the system design and the protocols developed for 80H2'dG in urine, plasma, cerebrospinal fluid, tissue, DNA, saliva, sweat, kidney dialysis fluid, foods, feces, culture matrix, and microdialysates. Concentrations in both human and animal body fluids and tissues are reported. The system performance is discussed in the context of a 1-year evaluation of the methods applied to approximately 3600 samples, using internal quality control and external blind testing to determine long-term accuracy. The methods are reliable and accurate, and therefore should prove useful in assessing the role and utility of oxidative DNA damage in aging and human illness. (C) 1999 Elsevier Science Inc.