Evidence for tissue-specific forms of glutaminyl cyclase
Evidence for tissue-specific forms of glutaminyl cyclase
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DOI：
10.1016/s0014-5793(99)00872-8
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发表时间：
1999-07-16
期刊：
影响因子：
3.5
通讯作者：
Bateman, RC
中科院分区：
文献类型：
--
作者：
Sykes, PA;Watson, SJ;Bateman, RC
谷氨酰环化酶（QC）是导致许多神经内分泌肽中焦谷氨酰残基存在的原因。通过对QC免疫反应性、酶活性和mRNA在牛组织分布的检测，证实QC在脑和垂体中大量存在。然而，酶活性比免疫反应性或mRNA广泛得多，表明有多种酶形式。部分纯化的牛脾脏QC与已知的牛垂体QC在物理和催化性能上有显著差异。我们提出，这种形式的谷氨酰环化酶在组成分泌的含葡聚糖蛋白的翻译后加工中起作用，(C) 1999年欧洲生化学会联合会。
Glutaminyl cyclase (QC) is responsible for the presence of pyroglutamyl residues in many neuroendocrine peptides, An examination of the bovine tissue distribution of QC immunoreactivity, enzyme activity, and mRNA confirmed that QC was abundant in brain and pituitary by all three measures. However, enzymatic activity was considerably more widespread than either immunoreactivity or mRNA, suggesting multiple enzyme forms. Partially purified QC from bovine spleen differed significantly from the known bovine pituitary QC in physical and catalytic properties. We propose that this form of glutaminyl cyclase plays a role in the posttranslational processing of constitutively secreted pGlu-containing proteins, (C) 1999 Federation of European Biochemical Societies.