Image correlation algorithm for measuring lymphocyte velocity and diameter changes in contracting microlymphatics
Image correlation algorithm for measuring lymphocyte velocity and diameter changes in contracting microlymphatics
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DOI：
10.1007/s10439-006-9225-2
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发表时间：
2007-03-01
影响因子：
3.8
通讯作者：
Cote, Gerard L.
中科院分区：
文献类型：
--
作者：
Dixon, J. Brandon;Gashev, Anatoliy A.;Cote, Gerard L.
最近，我们用高速视频显微镜系统对大鼠肠系膜血管收缩周期中的壁面切应力进行了研究。这是由文献中的报告提示的，即淋巴泵送与壁剪切应力有关。虽然人们可以通过跟踪淋巴细胞速度来估计壁面剪切应力，但是对于大量实验来说，在合理的时间范围内手动跟踪颗粒是非常繁琐的。为了克服这一点，一个图像相关方法类似于数字粒子成像测速仪的开发和测试上的收缩毛细管测量血管直径和流体速度。该程序跟踪空间平均速度的时间波动，预测标准误差为0.4 mm/s。从这些研究中，我们已经测量了范围从-2至4 mm/s的速度。直径的变化也测量了7 μ m的标准误差。这些算法和技术可能有利于研究收缩行为的各种变化作为速度和壁剪切应力变化的函数。
Efforts have recently been made to estimate wall shear stress throughout the contractile cycle of mesenteric rat lymphatics with a high speed video microscopy system. This was prompted by reports in the literature that lymphatic pumping is related to wall shear stress. While one can estimate wall shear stress by tracking lymphocyte velocity, it is prohibitively tedious to manually track particles over a reasonable time frame for a good number of experiments. To overcome this, an image correlation method similar to digital particle imaging velocimetry was developed and tested on contracting lymphatics to measure both vessel diameter and fluid velocity. The program tracked temporal fluctuations in spatially averaged velocity with a standard error of prediction of 0.4 mm/s. From these studies we have measured velocities ranging from -2 to 4 mm/s. Diameter changes were also measured with a standard error of 7 mu m. These algorithms and techniques could be beneficial for investigating various changes in contractile behavior as a function of changes in velocity and wall shear stress.