Induced pluripotent stem cell (iPSC) line (HEBHMUi002-A) from a healthy female individual and neural differentiation
Induced pluripotent stem cell (iPSC) line (HEBHMUi002-A) from a healthy female individual and neural differentiation
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来自健康女性个体的诱导多能干细胞 (iPSC) 系 (HEBHMUi002-A) 和神经分化。
DOI：
10.1016/j.scr.2019.101669
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发表时间：
2020-01-01
影响因子：
1.2
通讯作者：
Cui, Huixian
中科院分区：
文献类型：
--
作者：
Ma, Jun;Zhang, Jing;Cui, Huixian
诱导多能干细胞（iPSC）可用于在体外产生不同类型的体细胞，包括神经元细胞。在这里，人类 iPSC 系是从一名 39 岁健康个体的外周血单核细胞中产生的。所得 iPSC 不发生整合，保持正常核型，表达多能干细胞标记，并被证明能够分化为代表三个胚胎胚层的细胞。此外，我们表明该 iPSC 系可以分化为神经干细胞。总而言之，这种生成的 iPSC 系可用于测试多种分化方案，也可作为研究药物开发和疾病机制的对照。
Induced pluripotent stem cells (iPSCs) can be used to generate different types of somatic cells in vitro, including neuronal cells. Here, a human iPSC line was generated from the peripheral blood mononuclear cells of a healthy 39-year-old individual. The resulting iPSCs were integration-free, maintained the normal karyotype, expressed pluripotency stem cell markers, and were demonstrated to be capable of differentiating into cells representative of the three embryonic germ layers. Furthermore, we showed that this iPSC line could be differentiated into neural stem cells. Taken together, this generated iPSC line could be useful to test multiple differentiation protocols, and also serve as a control for investigating drug development and disease mechanisms.