Expression, localisation and phylogeny of a novel family of plant-specific membrane proteins
Expression, localisation and phylogeny of a novel family of plant-specific membrane proteins
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DOI：
10.1111/j.1438-8677.2010.00381.x
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发表时间：
2010-09-01
期刊：
影响因子：
3.9
通讯作者：
Kunze, R.
中科院分区：
文献类型：
--
作者：
Kasaras, A.;Kunze, R.
在拟南芥衰老相关基因的筛选中，鉴定了一种新的高度上调的膜蛋白。它是一个未表征的，严格的植物特异性基因家族的成员，被命名为AtDMP 1（拟南芥DUF 679域膜蛋白1）。预测Atomic蛋白具有四个跨膜跨度，具有胞质氨基和羧基末端。在这项研究中，我们研究了在A. thaliana.双子叶植物中的莱茵衣藻和小立碗藓基因组仅包含一个单拷贝的cDNA基因，而单子叶植物中有5 - 13个cDNA基因和11-16个cDNA基因，其中许多基因可能是最近的基因复制所致。在绿色植物中普遍存在的蛋白质和他们的缺席从其他王国建议在植物特异性过程中的作用。以.在拟南芥中，检测到10个中的9个表达。的表达模式被发现是显着的组织和发育特异性，因此，大多数蛋白质的功能冗余是不可能的。在经历衰老（AtDMP 1，-3，-4）、开裂（AtDMP 7）或脱落（AtDMP 2，-4，-7）的组织中出现几种AtDMPs表明DMPs参与不同类型的程序性细胞死亡。发现AtDMP-eGFP融合蛋白定位于内质网、液泡膜或在某些条件下定位于两种膜系统。进一步的研究正在进行中，以阐明Atoms蛋白的功能。
In a screen for senescence-associated genes in Arabidopsis thaliana, a novel, highly up-regulated membrane protein was identified. It is a member of an uncharacterised, strictly plant-specific gene family and was named AtDMP1 (Arabidopsis thaliana DUF679 domain membrane protein 1). The AtDMP proteins are predicted to have four transmembrane spans, with cytosolic amino- and carboxy-termini. In this study, we investigated the phylogenetic distribution of DMP proteins, their tissue-specific expression and subcellular localisation in A. thaliana. The Chlamydomonas reinhardtii and Physcomitrella patens genomes in dicots contain only a single DMP gene copy, whereas there are five to 13 DMP genes and 11-16 in monocots, many of which supposedly result from recent gene duplications. The ubiquitous occurrence of DMP proteins in green plants and their absence from other kingdoms suggest a role in plant-specific processes. In A. thaliana, expression of nine out of ten DMP genes was detected. The expression patterns were found to be markedly tissue- and development-specific; thus, functional redundancy of most proteins is unlikely. The occurrence of several AtDMPs in tissues undergoing senescence (AtDMP1, -3, -4), dehiscence (AtDMP7) or abscission (AtDMP2, -4, -7) suggests involvement of DMPs in different types of programmed cell death. AtDMP-eGFP fusion proteins were found to localise either to the endoplasmic reticulum, the tonoplast or, under certain conditions, to both membrane systems. Further investigations are in progress to elucidate functions of the AtDMP proteins.