Diversity and dynamics of free-living and particle-associated Betaproteobacteria and Actinobacteria in relation to phytoplankton and zooplankton communities
Diversity and dynamics of free-living and particle-associated Betaproteobacteria and Actinobacteria in relation to phytoplankton and zooplankton communities
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DOI：
10.1111/j.1574-6941.2011.01130.x
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发表时间：
2011-09-01
影响因子：
4.2
通讯作者：
Debroas, Didier
中科院分区：
文献类型：
--
作者：
Parveen, Bushra;Reveilliez, Jean-Philippe;Debroas, Didier
附着和自由生活的放线菌和Betaproteobacteria的多样性，16 S rRNA基因序列的基础上，在两个时期的对比浮游植物优势的中营养湖泊进行了研究。比较分析表明，附着和自由生活的两个细菌群体的社区之间的系统发育差异。对于Betaproteobacteria，在所有采样日期在自由生活和附着的细菌群落中检测到的betaI分支，是占主导地位的分支，占检索到的总操作分类单位（OTU）的57.8%。对于放线菌，acIV集群被认为是占主导地位的，其次是acI贡献的总检索OTU的45%和25%，分别。这项研究允许确定八个新的推定分支Betaproteobacteria之间的命名为lbI-lbVIII和一个新的推定分支命名acLBI属于放线菌。浮游植物和浮游动物群落的季节性动态反映为附着和自由生活群落的不同细菌群落类型的变化。对于附着群落，放线菌和蓝藻之间，betaproteobacteria和甲藻和绿藻生物量之间的关系进行了观察。另一方面，在自由生活的社区，很少放线菌分支被发现是依赖于营养物质或浮游植物群落，而Betaproteobacteria主要与生物参数（即浮游植物和桡足类群落）。
The diversity of attached and free-living Actinobacteria and Betaproteobacteria, based on 16S rRNA gene sequences, was investigated in a mesotrophic lake during two periods of contrasting phytoplankton dominance. Comparison analyses showed a phylogenetic difference between attached and free-living communities for the two bacterial groups. For Betaproteobacteria, the betaI clade was detected at all sampling dates in free-living and attached bacterial communities and was the dominant clade contributing to 57.8% of the total retrieved operational taxonomic units (OTUs). For Actinobacteria, the acIV cluster was found to be dominant, followed by acI contributing to 45% and 25% of the total retrieved OTUs, respectively. This study allows the determination of eight new putative clades among the Betaproteobacteria termed lbI-lbVIII and a new putative clade named acLBI belonging to the Actinobacteria. The seasonal dynamics of phytoplankton and zooplankton communities have been reflected as changes in distinct bacterial phylotypes for both attached and free-living communities. For attached communities, relationships were observed between Actinobacteria and Chrysophyceae, and between Betaproteobacteria and Dinophyceae and Chlorophyceae biomass. On the other hand, within free-living communities, few actinobacterial clades were found to be dependent on either nutrients or phytoplankton communities, whereas Betaproteobacteria were mainly associated with biological parameters (i.e. phytoplankton and copepod communities).