Next-generation sequencing analysis of lager brewing yeast strains reveals the evolutionary history of interspecies hybridization.
Next-generation sequencing analysis of lager brewing yeast strains reveals the evolutionary history of interspecies hybridization.
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啤酒酿造酵母菌菌株的下一代测序分析揭示了种间杂交的进化史。
DOI：
10.1093/dnares/dsv037
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发表时间：
2016-02
期刊：
影响因子：
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通讯作者：
Itoh T
中科院分区：
文献类型：
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作者：
Okuno M;Kajitani R;Ryusui R;Morimoto H;Kodama Y;Itoh T
贮藏啤酒酵母 Saccharomyces Pastorianus 被认为是 S. cerevisiae 和 S. eubayanus 之间的异源多倍体杂种。许多巴斯德酵母菌株已被分离出来，并根据地理起源分为两类，但这种分类仍然存在争议。杂交分析和部分基于 PCR 的序列数据表明这两个群体有不同的起源，而最近的易位分析表明有单一起源。为了阐明该物种的进化历史，我们通过 Illumina 新一代测序分析了 10 个巴斯德酵母菌株和作为可能亲本的 S. eubayanus 型菌株。除了组装其中五个菌株的基因组外，我们还获得了有关染色体间易位、倍性和单核苷酸变异 (SNV) 的信息。总的来说，这些结果表明两组菌株共享酿酒酵母单倍体染色体。因此，我们得出结论，两组巴斯德酵母菌株共享至少一个种间杂交事件，并且起源于共同的亲本物种，并且两组之间倍性和 SNV 的差异可以通过染色体缺失或杂合性丧失来解释。
The lager beer yeast Saccharomyces pastorianus is considered an allopolyploid hybrid species between S. cerevisiae and S. eubayanus. Many S. pastorianus strains have been isolated and classified into two groups according to geographical origin, but this classification remains controversial. Hybridization analyses and partial PCR-based sequence data have indicated a separate origin of these two groups, whereas a recent intertranslocation analysis suggested a single origin. To clarify the evolutionary history of this species, we analysed 10 S. pastorianus strains and the S. eubayanus type strain as a likely parent by Illumina next-generation sequencing. In addition to assembling the genomes of five of the strains, we obtained information on interchromosomal translocation, ploidy, and single-nucleotide variants (SNVs). Collectively, these results indicated that the two groups of strains share S. cerevisiae haploid chromosomes. We therefore conclude that both groups of S. pastorianus strains share at least one interspecific hybridization event and originated from a common parental species and that differences in ploidy and SNVs between the groups can be explained by chromosomal deletion or loss of heterozygosity.