Neural coding of quality of complex olfactory stimuli in lobsters.
Neural coding of quality of complex olfactory stimuli in lobsters.
复制标题
龙虾复杂嗅觉刺激质量的神经编码。
DOI：
10.1152/jn.1988.60.1.303
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发表时间：
1988
影响因子：
2.5
通讯作者：
Derby,CD
中科院分区：
文献类型：
--
作者：
Girardot,MN;Derby,CD
1. 研究了30个原代嗅细胞对复杂生物相关刺激的细胞外反应。刺激类型根据化学成分分为蟹、鲻鱼、牡蛎和虾的天然提取物和蟹、鲻鱼、牡蛎和虾的人工混合物。所有刺激类型均以0.005、0.05和0.5 mM三种浓度呈现。反应以每5秒的尖峰数表示。反应幅度随浓度的增加而显著增加。天然提取物的刺激强度显著高于相关的人工混合物，但在天然提取物和人工混合物的刺激类型之间差异不显著。3. 这些细胞对所有的刺激都有广泛的适应性。音调轻微但显著地随着刺激的集中而变宽。4. 基于人群反应，采用多维尺度（MDS）评价刺激之间的相似性和差异性。分别对人工混合物和天然提取物进行了分析。空间结构的维度基于以下三个标准：应力值、平方相关值和与质量编码的相关性。5. 当应用于原始数据时，MDS将刺激分布在二维空间中，其中每个刺激的位置主要基于刺激浓度。一个简单的标准化程序的结果表明，这种分布主要是由于浓度对人口响应的两个特征之一的显著影响，即绝对幅度。这个标准化程序使三种浓度在诱发反应的绝对强度方面相等。因此，可以仅根据它们的跨神经元模式（ANPs）（跨神经元的相对活动量）来比较12种刺激（4种类型X 3浓度）的神经群体反应。6. 当以应力和相关值的平方作为维数标准时，人工混合空间的构型最好从三维分辨率的1、2和3维中得到。当使用相关质量编码时，人工混合空间的构型最好由四维分辨率的1、3和4维导出。无论使用压力和平方相关值还是与质量编码的相关性，四种类型的刺激都占据不重叠的空间。（摘要删节为400字）
1. Extracellular responses to complex biologically relevant stimuli were recorded from 30 primary olfactory cells from excised antennules of spiny lobsters. The stimulus types were natural extracts of crab, mullet, oyster, and shrimp and artificial mixtures of crab, mullet, oyster and shrimp based on the chemical composition of the related extracts. All stimulus types were presented at the following three concentrations: 0.005, 0.05, and 0.5 mM. 2. The responses were expressed as number of spikes per 5 s. Response magnitude increased significantly as a function of concentration. It was significantly greater for the natural extracts than for the related artificial mixtures but was not significantly different among stimulus types within either natural extracts or artificial mixtures. 3. The cells were broadly tuned to all stimuli. Tuning slightly, but significantly, broadened as a function of stimulus concentration. 4. Multidimensional scaling (MDS) was used to evaluate similarities and dissimilarities among stimuli based on population responses. The artificial mixtures and the natural extracts were analyzed separately. Dimensionality of spatial configuration was based on the following three criteria: stress values, squared correlation values, and relevance to quality coding. 5. When applied to the original data, MDS distributed the stimuli in a two-dimensional space where the location of each stimulus was based mainly on stimulus concentration. The results of a simple standardization procedure showed that this distribution resulted mostly from the significant effect of concentration on one of the two features of population responses, which is the absolute magnitude. This standardization procedure equalized the three concentrations in terms of absolute magnitude of evoked response. Consequently, the neural population responses of the 12 stimuli (4 types X 3 concentrations) could be compared based only on their across-neuron patterns (ANPs) (relative amount of activity across neurons). 6. When stress and squared correlation values were used as criteria for dimensionality, the configuration of the artificial mixtures space was best derived from dimensions 1, 2, and 3 of the three-dimensional resolution. When relevance to quality coding was used, the configuration of the artificial mixtures space was best derived from dimensions 1, 3, and 4 of the four-dimensional resolution. Whether stress and squared correlation values or relevance to quality coding were used, the four types of stimuli occupied nonoverlapping spaces.(ABSTRACT TRUNCATED AT 400 WORDS)