Molecularly Ordered Bioelectrocatalytic Composite Inside a Film of Aligned Carbon Nanotubes
Molecularly Ordered Bioelectrocatalytic Composite Inside a Film of Aligned Carbon Nanotubes
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DOI：
10.1002/aenm.201200422
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发表时间：
2013
影响因子：
27.8
通讯作者：
S. Yoshino;T. Miyake;Takeo Yamada;K. Hata;M. Nishizawa
中科院分区：
文献类型：
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作者：
S. Yoshino;T. Miyake;Takeo Yamada;K. Hata;M. Nishizawa
将聚乙烯咪唑‐[Os（联吡啶）2Cl] （PVI‐[Os(bpy)2Cl]）和葡萄糖氧化酶（GOD）分子有序复合材料组装在排列整齐的碳纳米管薄膜内。制备的GOD/PVI‐[Os(bpy)2Cl]/CNT复合薄膜结构完全均匀稳定；即使在储存6天后，也能保持90%以上的生物电催化活性。由于酶、介质和电极之间实现了理想的位置关系，所制备的膜对葡萄糖氧化具有很高的生物电催化活性（25°C时约为15 mA cm - 2），具有极高的电子转移周转率（约650 s - 1），与GOD溶液的值相当。表明几乎每一种酶分子都被包裹在集合中（大约3 × 1012个酶在1毫米× 1毫米的膜中）可以发挥最大的作用。这种独立的柔性复合薄膜可以缠绕在针装置上使用；作为一个例子，我们演示了一个自供电的血糖监测仪。
Molecularly ordered composites of polyvinylimidazole‐[Os(bipyridine)2Cl] (PVI‐[Os(bpy)2Cl]) and glucose oxidase (GOD) are assembled inside a film of aligned carbon nanotubes. The structure of the prepared GOD/PVI‐[Os(bpy)2Cl]/CNT composite film is entirely uniform and stable; more than 90% bioelectrocatalytic activity could be maintained even after storage for 6 d. Owing to the ideal positional relationship achieved between enzyme, mediator, and electrode, the prepared film shows a high bioelectrocatalytic activity for glucose oxidation (ca. 15 mA cm−2 at 25 °C) with an extremely high electron‐transfer turnover rate (ca. 650 s−1) comparable to the value for GOD solutions, indicating almost every enzyme molecule entrapped within the ensemble (ca. 3 × 1012 enzymes in a 1 mm × 1 mm film) can work to the fullest extent. This free‐standing, flexible composite film can be used by winding on a needle device; as an example, a self‐powered sugar monitor is demonstrated.