Antinociceptive effect of intracerebroventricular administration of d-serine on formalin-induced pain
Antinociceptive effect of intracerebroventricular administration of d-serine on formalin-induced pain
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DOI：
10.1007/s00540-013-1708-3
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发表时间：
2014-04
影响因子：
2.8
通讯作者：
Miho Ito;M. Yoshikawa;Kenji Ito;M. Matsuda;X. Jin;Shigeru Takahashi;Hiroyuki Kobayashi;Toshiyasu Suzuki
中科院分区：
文献类型：
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作者：
Miho Ito;M. Yoshikawa;Kenji Ito;M. Matsuda;X. Jin;Shigeru Takahashi;Hiroyuki Kobayashi;Toshiyasu Suzuki
目的在先前的甩尾实验中，我们发现侧脑室注射N-甲基-D-天冬氨酸(NMDA)受体甘氨酸位点的内源性协同激动剂d-丝氨酸对热伤害性感受具有抗伤害性作用。本研究旨在通过福尔马林实验评价侧脑室注射d-丝氨酸对组织损伤或炎症所致伤害性反应的影响。以10μ的体积输注药物2分钟以上，并将输液插管放置2分钟后取出。结果侧脑室注射d-丝氨酸可显著减少福尔马林试验早期和晚期的甩尾数目，并呈剂量依赖关系。这种抗伤害性作用可被脑室注射N-甲基-D-天冬氨酸受体甘氨酸位点拮抗剂L-701,324所拮抗。结论脊髓上水平甘氨酸位点激活N-甲基-D-天冬氨酸受体对急性疼痛和紧张性疼痛均有抗伤害效应。
PurposeIn a previous study using the tail-flick test, we found that intracerebroventricular administration ofd-serine, an endogenous co-agonist at the glycine sites ofN-methyl-d-aspartate (NMDA) receptors, elicited an antinociceptive effect on thermal nociception. The purpose of the present study was to evaluate the effect of intracerebroventricular administration ofd-serine on nociception induced by tissue damage or inflammation using the formalin test.MethodsInfusion of drugs into the third ventricle in rat was performed via indwelling cannulae. Drugs were infused at a volume of 10 μl over 2 min, and the infusion cannula was left in place for 2 min before removal. The formalin test was performed 10 min after drug administration.ResultsIntracerebroventricular administration ofd-serine significantly and dose-dependently decreased the number of flinches in both the early and late phases in the formalin test. This antinociceptive effect was antagonized by intracerebroventricular administration of L-701,324, a selective antagonist at the glycine sites of NMDA receptors.ConclusionThe present data suggest that activation of NMDA receptors via glycine sites at the supraspinal level induces an antinociceptive effect on both acute and tonic pain.