Microglia support neural stem cell maintenance and growth
Microglia support neural stem cell maintenance and growth
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DOI：
10.1016/j.bbrc.2018.07.130
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发表时间：
2018-09-10
影响因子：
3.1
通讯作者：
Mori, Eiichiro
中科院分区：
文献类型：
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作者：
Matsui, Takeshi K.;Mori, Eiichiro
越来越多的证据表明，与疾病相关的小胶质细胞在神经退行性疾病中发挥着保护作用。已知小胶质细胞响应外部信号炎症 M1 状态和抗炎 M2 状态而极化成两种往复形式。这些细胞在大脑发育中发挥关键功能，例如电路细化、神经发生和神经元生长。在本研究中，我们分析了小胶质细胞的分泌对神经干/祖细胞（NSPC）增殖和分化的影响。我们在不含生长因子的 BV2 永生化小胶质细胞的条件培养基中培养了成年小鼠衍生的 NSPC，并评估了它们的分化。当在碱性成纤维细胞生长因子 (bFGF) 和表皮生长因子 (EGF) 存在的情况下用 BV2 衍生的可溶性因子培养时，与仅用 bFGF 和 EGF 培养的细胞相比，NSPC 能够维持巢蛋白表达，并显示出增殖增加。此外，来自 M2 极化原代小胶质细胞的条件培养基在 IL-10/IL-13 刺激下显示出对 NSPC 增殖的支持作用。这些数据表明小胶质细胞通过分泌神经营养可溶性因子支持神经干细胞增殖。 (C) 2018 作者。由爱思唯尔公司出版
Increasing evidence suggests that disease-associated microglia play a protective role in neurodegenerative diseases. Microglia are known to polarize into two reciprocate forms in response to external cues inflammatory M1 state and anti-inflammatory M2 state. These cells perform key functions in the development of the brain, such as circuit refinement, neurogenesis, and neuronal growth. In this study, we analyzed the secretion effect of microglia on neural stem/progenitor cell (NSPC) proliferation and differentiation. We cultured adult mouse-derived NSPCs in a conditioned medium from BV2 immortalized microglia without growth factors and evaluated their differentiation. When cultivated with BV2-derived soluble factors in the presence of basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF), NSPCs were able to maintain Nestin expression and showed increased proliferation compared with those cells cultivated with bFGF and EGF only. Moreover, conditioned media from M2-polarized primary microglia, stimulated by IL-10/IL-13, showed supportive effect on NSPC proliferation. These data suggest that microglia support neural stem cell proliferation through secreting neuronutritious soluble factors. (C) 2018 The Authors. Published by Elsevier Inc.