Identification of a second β chain in pig brain tubulin
Identification of a second β chain in pig brain tubulin
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猪脑微管蛋白第二条β链的鉴定
DOI：
10.1016/0014-5793(79)81229-6
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发表时间：
1979
期刊：
影响因子：
3.5
通讯作者：
M. Little
中科院分区：
文献类型：
--
作者：
M. Little
微管是纤毛、鞭毛和有丝分裂器的主要结构成分。它们还参与形态发生、细胞定向、细胞内运输和分泌过程[11]。微管的结构单元，微管蛋白，通常被认为是一个异源二聚体的Y和O链，每个摩尔。wt-55 000 [2]。然而，最近，在从埃利希腹水肿瘤细胞和猪脑中纯化的微管蛋白制剂中发现了第三种组分，其在给定条件下在SDS-聚丙烯酰胺凝胶电泳上在β和β微管蛋白之间迁移[3]。这种蛋白质可能代表微管系统的一种特殊组分，在此，猪脑微管蛋白制剂中的第三种微管蛋白组分被鉴定为第二条/3链。一种改进的微管蛋白（Y和O链）制备分离方法。微管蛋白亚基的特征在于有限的蛋白水解和肽图谱。
Microtubules are the main structural components of cilia, flagella and the mitotic apparatus. They also participate in morphogenesis, cell orientation, intracellular transport and secretion processes [11. The building block of microtubules, the protein tubulin, is generally assumed to be a heterodimer of (Y and 0 chains, each of mol. wt-55 000 [2]. Recently, however, a third component has been found in purified tubulin preparations from both Ehrlich ascites tumour cells and pig brain which migrates between the (Y and fl tubulins on SDS-polyacrylamide gel electrophoresis under given conditions [3]. It is possible that this protein represents a specific component of the microtubule system.Here, the third tubulin component in a preparation of tubulin from pig brain has been identified as a second/3 chain. An improved procedure for the preparative isolation of tubulin (Y and 0 chains is. described, and the tubulin subunits have been characterised by limited proteolysis and peptide mapping.