Efficacy of ultraviolet light-emitting diodes (UV-LED) at four different peak wavelengths against Cryptosporidium parvum oocysts by inactivation assay using immunodeficient mice
Efficacy of ultraviolet light-emitting diodes (UV-LED) at four different peak wavelengths against Cryptosporidium parvum oocysts by inactivation assay using immunodeficient mice
复制标题
使用免疫缺陷小鼠进行灭活试验，研究四种不同峰值波长的紫外线发光二极管 (UV-LED) 对小隐孢子虫卵囊的功效
DOI：
10.1016/j.parint.2020.102108
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发表时间：
2020
影响因子：
1.9
通讯作者：
Teramoto Isao
中科院分区：
文献类型：
--
作者：
Takahashi Karin;Matsubayashi Makoto;Ohashi Yukio;Naohara Jun;Urakami Itsuo;Sasai Kazumi;Kido Yasutoshi;Kaneko Akira;Teramoto Isao
作为使用紫外线(UV)灯的替代方案，紫外线发光二极管(UV-LED)有望有效地灭活水中的微生物。紫外线灯由对环境和人类健康有害的汞制成。虽然UV-LED已经被报道对细菌和病毒有效，但UV-LED对隐孢子虫寄生虫的有效性还没有得到充分的评估。正如我们在这里报道的，我们已经开发出一种活体定量失活分析方法。使用免疫缺陷小鼠的微小卵囊。使用该方法，我们评估了大约1000GμJ/cm~2的紫外灯(距离为95 mm的S 3)与商用UV-LED(峰值波长为268、275、284和289 nm)灭活的效果。与紫外灯处理相比，284和289 nm波长的UV-LED处理后的卵囊脱落模式明显延迟。这些发现提供了第一个建议，即UV-LED对这些寄生虫有效，正如在固定曝光距离和时间条件下使用商用350毫安UV-LED进行评估一样。
As an alternative to using ultraviolet (UV) lamps, which are made with mercury that is toxic to the environment and human health, UV light-emitting diodes (UV-LEDs) are expected to be effective for inactivating microorganisms in water. Although UV-LEDs have been reported to be effective against bacteria and viruses, the effectiveness of UV-LEDs againstCryptosporidiumparasites has not been fully evaluated. As we report here, we have developed anin vivoquantitative inactivation assay forC. parvumoocysts using immunodeficient mice. Using the assay, we evaluated the effectiveness of treatment by UV lamp (254 nm) at approximately 1000 μJ/cm2(for 3 s at a distance of 95 mm) compared to inactivation by commercially available UV-LEDs (with peak wavelengths of 268, 275, 284, and 289 nm). The shed patterns of oocysts after treatment with 284- and 289-nm wavelength UV-LEDs were significantly delayed compared to that after treatment with a UV lamp. These findings provide the first suggestion that UV-LEDs are effective against these parasites, as assessed using commercially available 350-mA UV-LEDs under conditions of fixed exposure distance and time.