Terminal loop-mediated regulation of miRNA biogenesis: selectivity and mechanisms.
Terminal loop-mediated regulation of miRNA biogenesis: selectivity and mechanisms.
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DOI：
10.1042/bst20130058
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发表时间：
2013-08
影响因子：
3.9
通讯作者：
Ramos A
中科院分区：
文献类型：
--
作者：
Castilla-Llorente V;Nicastro G;Ramos A
调节单个miRNAs（microRNAs）的表达对细胞发育和功能非常重要。特定miRNA前体加工成成熟活性形式的上调或下调代表了控制miRNA浓度的一种工具，并且由识别RNA前体末端环的蛋白质介导。末端环识别是通过几个RNA结合结构域的联合作用实现的。然后，蛋白质可以通过募集RNA酶、改变RNA结构以及防止或增强核心加工复合物的可接近性和加工活性来调节加工。本综述着重于末端环结合蛋白如何识别其RNA靶点并介导其调控功能，并强调末端环介导的调控如何与更广泛的mRNA代谢调控相关。
Regulating the expression of individual miRNAs (microRNAs) is important for cell development and function. The up- or down-regulation of the processing of specific miRNA precursors to the mature active form represents one tool to control miRNA concentration and is mediated by proteins that recognize the terminal loop of the RNA precursors. Terminal loop recognition is achieved by the combined action of several RNA-binding domains. The proteins can then regulate the processing by recruiting RNA enzymes, changing the RNA structure and preventing or enhancing the accessibility and processing activity of the core processing complexes. The present review focuses on how terminal loop-binding proteins recognize their RNA targets and mediate their regulatory function(s), and highlights how terminal loop-mediated regulation relates to the broader regulation of mRNA metabolism.