Temperature selectivity in reversed-phase high performance liquid chromatography
Temperature selectivity in reversed-phase high performance liquid chromatography
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DOI：
10.1016/s0021-9673(01)01321-8
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发表时间：
2002-08-02
影响因子：
4.1
通讯作者：
Dolan, JW
中科院分区：
文献类型：
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作者：
Dolan, JW
柱温在反相高效液相色谱（RP-HPLC）中起着两个重要的作用：控制保留度(k)和控制选择性（alpha）。虽然保留随温度的变化是普遍存在的，但对于任何给定的溶质对的选择性变化对于电离样品和具有更多极性取代基的样品更为明显。对于许多样品，可以以优化分辨率的方式选择柱温。在RP-HPLC中观察到的温度变化的选择性效应通常与流动相强度变化的选择性效应是互补的，因此通常可以通过同时优化温度和流动相有机百分比或梯度陡峭度来提高分辨率。计算机模拟是进行这类优化实验的有力工具。综述了温度对反相高效液相色谱选择性的影响。(C) 2002 Elsevier Science B.V.版权所有
Column temperature plays two important roles in reversed-phase high-performance liquid chromatography (RP-HPLC): control of retention (k) and control of selectivity (alpha). While changes in retention as a function of temperature are ubiquitous, selectivity changes for any given solute pair are more pronounced for ionized samples and samples with more polar substituents. With many samples, column temperature can be selected in a manner that optimizes resolution. The selectivity effects observed for temperature changes in RP-HPLC generally are complementary to those observed for mobile phase strength changes, so it is often possible to improve resolution by simultaneous optimization of temperature and mobile phase percent organic or gradient steepness. Computer simulation is a powerful tool for such optimization experiments. This paper reviews the influence of temperature on chromatographic selectivity for RP-HPLC. (C) 2002 Elsevier Science B.V. All rights reserved.