A new method for the preparation of three-layer vascular stents: a preliminary study on the preparation of biomimetic three-layer vascular stents using a three-stage electrospun membrane
A new method for the preparation of three-layer vascular stents: a preliminary study on the preparation of biomimetic three-layer vascular stents using a three-stage electrospun membrane
复制标题
三层血管支架制备新方法：三级电纺膜制备仿生三层血管支架的初步研究
DOI：
10.1088/1748-605x/ab920a
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发表时间：
2020-09-01
影响因子：
4
通讯作者：
Zhang, Haibo
中科院分区：
文献类型：
--
作者：
Chen, Xinxin;Chen, Dian;Zhang, Haibo
目前迫切需要设计一种具有良好生物相容性和足够机械强度的组织工程血管移植物来修复和促进缺陷血管组织的再生。人们普遍认为多层支架可以模拟天然血管的结构和功能。在这里，我们开发了一种新的三层管状接枝材料，该接枝材料是由单聚l -乳酸-co-己内酯电纺丝膜轧制而成的。我们使用了一种新的静电纺丝技术，在一个静电纺丝膜上放置了三种不同的结构，这样支架就由三层不同的结构组成。内层致密，适合内皮细胞生长，中间层为平行松散结构，适合平滑肌细胞生长，外层为平行稀疏交替结构，既适合平滑肌细胞生长，又适合结构支撑。血管支架具有良好的抗拉强度。同时，内皮细胞和平滑肌细胞在该材料上容易增殖。特别是，平滑肌细胞在中、外材料上平行生长。在体内，血管移植物的所有层在皮下植入一周内被细胞浸润，表明良好的生物相容性。皮下植入1周后，将血管支架原位植入大鼠腹主动脉。移植10周后，腹部超声成像显示血管通畅。血管支架内皮化，平滑肌细胞容易增殖，并形成大量弹性纤维。因此，我们特别设计的三层血管移植物可能对血管重建有显著的好处。
There is an urgent need to design a tissue-engineered vascular graft that exhibits good biocompatibility and sufficient mechanical strength to repair and facilitate regeneration of defective vascular tissue. It is generally accepted that multi-layer stents can be used to simulate the structure and function of natural blood vessels. Here, we developed a new three-layer tubular graft that is rolled from a single Poly(L-lactide-co-caprolactone) electrospun membrane. We used a new electrospinning technique to place three different structures on a single electrospun membrane such that the stent is comprised of three different layers. The inner layer is dense and suitable for endothelial cell growth, the middle layer is a parallel loose structure suitable for smooth muscle cell growth, and the outer layer is a parallel structure with sparse alternating texture suitable for both smooth muscle cell growth and structural support. The vascular stent has good tensile strength. At the same time, endothelial cells and smooth muscle cells readily proliferate on the material in vitro. In particular, smooth muscle cells grow in parallel on the middle and outer materials. In vivo, all layers of the vascular graft were infiltrated by cells within one week of subcutaneous implantation, indicative of favorable biocompatibility. After a week of subcutaneous implantation, the vascular stent was orthotopically transplanted into the abdominal aorta of Sprague Dawley rats. After ten weeks of transplantation, ultrasound imaging of the abdomen showed vascular patency. The vascular stent was endothelialized, smooth muscle cells readily proliferated, and a large amount of elastic fibers were formed. Therefore, our specially designed tri-layer vascular graft may be of significant benefit in vascular reconstruction.