HORMONE THERAPY AND MAXIMAL ECCENTRIC EXERCISE ALTERS MYOSTATIN-RELATED GENE EXPRESSION IN POSTMENOPAUSAL WOMEN
HORMONE THERAPY AND MAXIMAL ECCENTRIC EXERCISE ALTERS MYOSTATIN-RELATED GENE EXPRESSION IN POSTMENOPAUSAL WOMEN
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DOI：
10.1519/jsc.0b013e318251083f
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发表时间：
2012-05-01
影响因子：
3.2
通讯作者：
Schroeder, E. Todd
中科院分区：
文献类型：
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作者：
Dieli-Conwright, Christina M.;Spektor, Tanya M.;Schroeder, E. Todd
Dieli-Conwright, CM、Spektor, TM、Rice, JC、Sattler, FR 和 Schroeder, ET。激素治疗和最大离心运动改变绝经后女性肌生长抑制素相关基因的表达。 J Strength Cond Res 26(5): 1374-1382, 2012 - 我们试图评估接受激素治疗 (HT) 和在离心运动后不接受 HT 的绝经后妇女的基线 mRNA 值和肌生长抑制素相关因子的基因表达变化。 14 名绝经后女性参与其中，包括 6 名未使用 HT 的对照者（59 +/- 4 岁，63 +/- 17 公斤）和 8 名使用 HT 的女性（59 +/- 4 岁，89 +/- 24 公斤）。参与者在测力计上进行 10 组，每组 10 次最大偏心重复单腿伸展。股外侧肌的肌肉活检是在基线时和锻炼后 4 小时从锻炼的腿部获得的。使用逆转录酶聚合酶链反应测定肌生长抑制素、激活素受体 IIb (ActRIIb)、卵泡抑素、卵泡抑素相关基因 (FLRG)、卵泡抑素样 3 (FSTL3) 和 GDF 血清相关蛋白 1 (GASP-1) 的基因表达。作为对运动的反应，两组的肌生长抑制素和 ActRIIb 均显着下降 (p < 0.05)，而卵泡抑素、FLRG、FSTL3 和 GASP-1 均显着增加 (p < 0.05)。急性离心运动后，HT 组所有基因的基因表达变化均显着大于对照组（p < 0.05）。这些数据表明，使用 HT 的绝经后女性的肌生长抑制素相关基因表达更高，这可能反映了雌激素影响肌肉质量保存的机制。此外，使用 HT 的绝经后女性对最大离心运动的肌生长抑制素相关反应明显增强。
Dieli-Conwright, CM, Spektor, TM, Rice, JC, Sattler, FR, and Schroeder, ET. Hormone therapy and maximal eccentric exercise alters myostatin-related gene expression in postmenopausal women. J Strength Cond Res 26(5): 1374-1382, 2012-We sought to evaluate baseline mRNA values and changes in gene expression of myostatin-related factors in postmenopausal women taking hormone therapy (HT) and not taking HT after eccentric exercise. Fourteen postmenopausal women participated including 6 controls not using HT (59 +/- 4 years, 63 +/- 17 kg) and 8 women using HT (59 +/- 4 years, 89 +/- 24 kg). The participants performed 10 sets of 10 maximal eccentric repetitions of single-leg extension on a dynamometer. Muscle biopsies from the vastus lateralis were obtained from the exercised leg at baseline and 4 hours after the exercise bout. Gene expression was determined using reverse transcriptase polymerase chain reaction for myostatin, activin receptor IIb (ActRIIb), follistatin, follistatin-related gene (FLRG), follistatin-like-3 (FSTL3), and GDF serum-associated protein-1 (GASP-1). In response to the exercise bout, myostatin and ActRIIb significantly decreased (p < 0.05), and follistatin, FLRG, FSTL3, and GASP-1 significantly increased in both groups (p < 0.05). Significantly greater changes in gene expression of all genes occurred in the HT group than in the control group after the acute eccentric exercise bout (p < 0.05). These data suggest that postmenopausal women using HT express greater myostatin-related gene expression, which may reflect a mechanism by which estrogen influences the preservation of muscle mass. Further, postmenopausal women using HT experienced a profoundly greater myostatin-related response to maximal eccentric exercise.