A link between nuclear RNA surveillance, the human exosome and RNA polymerase II transcriptional termination.
A link between nuclear RNA surveillance, the human exosome and RNA polymerase II transcriptional termination.
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DOI：
10.1093/nar/gkq703
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发表时间：
2010-12
影响因子：
14.9
通讯作者：
Carmo-Fonseca M
中科院分区：
文献类型：
--
作者：
de Almeida SF;García-Sacristán A;Custódio N;Carmo-Fonseca M
在真核生物中，成熟信使RNA的产生离开细胞核以在细胞质中翻译成蛋白质，需要对新生转录物进行精确和广泛的修饰。任何损害mRNA完整性的失败都可能导致其保留在细胞核中并引发其降解。多项研究表明，具有加工缺陷的mRNA积累在位于转录位点附近的核灶或“点”中，但缺陷RNA如何被识别和束缚仍然是未知的。在这里，我们提出的证据表明，未加工的β-珠蛋白转录使RNA聚合酶II（Pol II）不能终止，这种质量控制过程需要完整的核外泌体。我们的研究结果表明，未加工的前mRNA仍然拴在DNA模板与Pol II，在Rrp 6依赖的方式。这揭示了核RNA监视、外泌体和Pol II转录终止之间前所未有的联系。
In eukaryotes, the production of mature messenger RNA that exits the nucleus to be translated into protein in the cytoplasm requires precise and extensive modification of the nascent transcript. Any failure that compromises the integrity of an mRNA may cause its retention in the nucleus and trigger its degradation. Multiple studies indicate that mRNAs with processing defects accumulate in nuclear foci or ‘dots’ located near the site of transcription, but how exactly are defective RNAs recognized and tethered is still unknown. Here, we present evidence suggesting that unprocessed β-globin transcripts render RNA polymerase II (Pol II) incompetent for termination and that this quality control process requires the integrity of the nuclear exosome. Our results show that unprocessed pre-mRNAs remain tethered to the DNA template in association with Pol II, in an Rrp6-dependent manner. This reveals an unprecedented link between nuclear RNA surveillance, the exosome and Pol II transcriptional termination.
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影响因子：
14.9
作者：
Callahan KP;Butler JS
通讯作者：
Butler JS
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作者：
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