The collection of high-resolution electron diffraction data.
The collection of high-resolution electron diffraction data.
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DOI：
10.1007/978-1-62703-176-9_9
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发表时间：
2013-01-01
期刊：
影响因子：
--
通讯作者：
Gonen, Tamir
中科院分区：
文献类型：
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作者：
Gonen, Tamir
最近用电子结晶学测定了一些膜蛋白的原子分辨结构(优于3A分辨)。虽然这项技术建立在40多年前，但在二维(2D)结晶、数据收集、数据分析和蛋白质结构确定方面仍处于起步阶段。在数据采集方面，电子结晶学包括图像采集和电子衍射数据采集。本卷的其他章节概述了图像采集和分析的协议。然而，这一章概述了通过电子衍射收集数据的详细协议。其中包括显微镜设置、电子衍射数据收集和故障排除。
A number of atomic-resolution structures of membrane proteins (better than 3A resolution) have been determined recently by electron crystallography. While this technique was established more than 40 years ago, it is still in its infancy with regard to the two-dimensional (2D) crystallization, data collection, data analysis, and protein structure determination. In terms of data collection, electron crystallography encompasses both image acquisition and electron diffraction data collection. Other chapters in this volume outline protocols for image collection and analysis. This chapter, however, outlines detailed protocols for data collection by electron diffraction. These include microscope setup, electron diffraction data collection, and troubleshooting.