Ex vivo fluorescence molecular tomography of the spine.
Ex vivo fluorescence molecular tomography of the spine.
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DOI：
10.1155/2012/942326
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发表时间：
2012
影响因子：
7.6
通讯作者：
Intes X
中科院分区：
文献类型：
--
作者：
Pimpalkhare M;Chen J;Venugopal V;Intes X
我们研究了荧光分子断层扫描成像从大型动物模型中收集的离体样品的潜力，在这种情况下，狗脊柱。宽视场时间选通荧光断层扫描被用来评估多视图采集、数据类型和内在光学特性对椎间盘中荧光夹杂物成像的定位和定量准确性的影响。正如预期的那样，TG数据集在结合早期和晚期门时，在定位和量化方面提供比CW数据集明显更好的性能。此外，多视图成像协议的使用导致更准确的定位。此外，将组织的异质性质并入模型中以计算雅可比矩阵导致改进的成像性能。这个初步的成像研究提供了一个概念的可行性证明，定量成像复杂的离体样品非破坏性和短的采集时间。这项工作是第一步，采用光学分子成像的脊柱检测和表征椎间盘退变的基础上有针对性的荧光探针。
We investigated the potential of fluorescence molecular tomography to image ex vivo samples collected from a large animal model, in this case, a dog spine. Wide-field time-gated fluorescence tomography was employed to assess the impact of multiview acquisition, data type, and intrinsic optical properties on the localization and quantification accuracy in imaging a fluorescent inclusion in the intervertebral disk. As expected, the TG data sets, when combining early and late gates, provide significantly better performances than the CW data sets in terms of localization and quantification. Moreover, the use of multiview imaging protocols led to more accurate localization. Additionally, the incorporation of the heterogeneous nature of the tissue in the model to compute the Jacobians led to improved imaging performances. This preliminary imaging study provides a proof of concept of the feasibility of quantitatively imaging complex ex vivo samples nondestructively and with short acquisition times. This work is the first step towards employing optical molecular imaging of the spine to detect and characterize disc degeneration based on targeted fluorescent probes.