Clinical helminthiases in Thailand border regions show elevated prevalence levels using qPCR diagnostics combined with traditional microscopic methods
Clinical helminthiases in Thailand border regions show elevated prevalence levels using qPCR diagnostics combined with traditional microscopic methods
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DOI：
10.1186/s13071-020-04290-0
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发表时间：
2020-08-12
影响因子：
3.2
通讯作者：
Gobert, Geoffrey N.
中科院分区：
文献类型：
--
作者：
Adisakwattana, Poom;Yoonuan, Tippayarat;Gobert, Geoffrey N.
背景：东南亚国家边境管制不足是寄生虫传播增强的潜在地区，也是寄生虫病控制的障碍。方法：对泰国边境靠近缅甸、老挝和柬埔寨的三个地区进行临床寄生虫病调查。对567人的粪便进行了现场显微镜检查。结果：经Kato-Katz法和qPCR法评估，总蠕虫感染率分别为17.99%和24.51%。两种方法联合检测的患病率为28.57%，检出的优势种为间日圆锥虫(18.34%)、钩虫(6.88%)和钩虫(6.88%)。结论：分子诊断学对更准确地确定东南亚地区蠕虫硫酶的流行水平具有重要价值。提供这种准确的流行率信息将有助于指导未来的公共卫生倡议，并凸显在这些地区进行更严格的监测和及时干预的必要性。
Background: Under-regulated national borders in Southeast Asia represent potential regions for enhanced parasitic helminth transmission and present barriers to helminthiasis disease control.Methods: Three Thailand border regions close to Myanmar, Laos and Cambodia were surveyed for clinical parasitic helminth disease. In-field microscopy was performed on stools from 567 individuals. Sub-samples were transported to Bangkok for molecular analysis comprising three multiplex qPCR assays.Results: The overall helminth infection prevalence was 17.99% as assessed by Kato-Katz and 24.51% by qPCR. The combined prevalence of the two methods was 28.57%; the most predominant species detected wereOpisthorchis viverrini(18.34%), hookworm (6.88%;Ancylostomaspp. andNecator americanus),Ascaris lumbricoides(2.29%) andTrichuris trichiura(1.76%).Conclusions: These data demonstrate the value of molecular diagnostics for determining more precise prevalence levels of helminthiases in Southeast Asia. Availability of such accurate prevalence information will help guide future public health initiatives and highlights the need for more rigorous surveillance and timely intervention in these regions.