PHARMACOLOGICAL DISSECTION OF PYLORIC NETWORK OF THE LOBSTER STOMATOGASTRIC GANGLION USING PICROTOXIN
PHARMACOLOGICAL DISSECTION OF PYLORIC NETWORK OF THE LOBSTER STOMATOGASTRIC GANGLION USING PICROTOXIN
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DOI：
10.1152/jn.1980.44.6.1089
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发表时间：
1980-01-01
影响因子：
2.5
通讯作者：
BIDAUT, M
中科院分区：
文献类型：
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作者：
BIDAUT, M
印防己毒素（PTX）（10-7-10-6 M）完全阻断龙虾（P. interruptus）口胃神经节幽门模式发生器中的大多数抑制性突触。检查了来自大多数类别的已识别神经元的突触的敏感性。长时间洗涤至少可以部分逆转阻塞。来自幽门扩张器（PD）神经元的突触是唯一的抑制性突触，印防己毒素未能完全阻断。一个相关性推导出，短暂的，快速上升的抑制性突触后电位（IPSP）是印防己毒素敏感，而一个缓慢的圆形组成部分的IPSP从PD神经元是不印防己毒素敏感。印防己毒素引起由16细胞幽门网络产生的运动节律模式的特定变化。这揭示了幽门发电机中特定突触的功能作用。驱动幽门节律的内源性爆发神经元（PD和前爆发）保持相似的爆发率。在印防己毒素作用下，幽门跟随神经元相对于驱动神经元均向后相移动。一些正常的拮抗细胞，相关的相互抑制连接，成为同相。这些和其他模式的变化可能与特定突触的阻滞有关。尽管突触网络的回路发生了巨大的变化，但在印防己毒素的作用下，幽门节律仍然很明显。这支持了PD驱动神经元的周期性抑制在产生幽门模式中起主要作用的观点。
Picrotoxin (PTX) (10-7-10-6 M) completely blocked most inhibitory synapses in the pyloric pattern generator of the lobster (P. interruptus) stomatogastric ganglion. The sensitivity of synapses from most classes of identified neurons was examined. Blockade was at least partly reversible with prolonged washing. The synapses from pyloric dilator (PD) neurons were the only inhibitory synapses that picrotoxin failed to block completely. A correlation is derived that brief, fast-rise inhibitory postsynaptic potentials (IPSP) are picrotoxin sensitive, whereas a slow rounded component of IPSP from PD neurons is not picrotoxin sensitive. Picrotoxin caused specific changes in the pattern of the motor rhythm produced by the 16-cell pyloric network. This sheds some light on the functional role of particular synapses in the pyloric generator. The endogenously bursting neurons (PD and anterior burster), which drive the pyloric rhythm, kept a similar burst rate. Under picrotoxin, the pyloric follower neurons all moved to later phase relative to the driver group. Some normally antagonistic cells, related by reciprocal inhibitory connections, became inphase. These and other pattern changes could be related to blockade of particular synapses. The pyloric rhythm was still quite recognizable under picrotoxin despite the drastically altered circuitry of the synaptic network. This supports the idea that periodic inhibition from the PD driver neurons plays a primary role in creating the pyloric pattern.