Growth regulation of the expression of mouse cDNA and gene encoding a serine/threonine kinase related to Saccharomyces cerevisiae CDC7 essential for G1/S transition -: Structure, chromosomal localization, and expression of mouse gene for S-cerevisiae CDC7-related kinase
Growth regulation of the expression of mouse cDNA and gene encoding a serine/threonine kinase related to Saccharomyces cerevisiae CDC7 essential for G1/S transition -: Structure, chromosomal localization, and expression of mouse gene for S-cerevisiae CDC7-related kinase
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DOI：
10.1074/jbc.273.36.23248
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发表时间：
1998-09-04
影响因子：
4.8
通讯作者：
Masai, H
中科院分区：
文献类型：
--
作者：
Kim, JM;Sato, N;Masai, H
酿酒酵母CDC 7编码酵母细胞G（1）/S转换所需的丝氨酸/苏氨酸激酶。我们以前报告了人类和非洲爪蟾的cDNA编码CDC 7相关激酶，并建议的可能性，高等真核生物的染色体复制可能是通过涉及Cdc 7相关激酶的保守机制进行调节。在这里，我们报告了小鼠的cDNA和基因（muCdc 7）编码的丝氨酸/苏氨酸激酶相关的CDC 7。muCdc 7最长cDNA的预测编码框架由564个氨基酸组成，与芽殖酵母、非洲爪蟾和人类的激酶保守结构域分别具有46%、77%和93%的同一性。muCdc 7的染色体基因位于小鼠5号染色体上的5E 5带，由12个外显子组成，其外显子/内含子组织与包括Cdk和cAMP依赖性激酶在内的其他蛋白激酶的组织有一定的相似性。muCdc 7的转录起始于370个碱基对启动子区域的多个位点，在静息状态下受到抑制，并在生长因子依赖性细胞系中生长因子刺激后在G（1）/S边界处被诱导。瞬时转染实验表明，一个231碱基对的muCdc 7启动子片段含有三个推定的E2 F结合位点和一个Sp1位点，但缺乏TATA序列是足够的响应生长刺激。
Saccharomyces cerevisiae CDC7 encodes a serine/threonine kinase required for G(1)/S transition of the yeast cells. We previously reported human and Xenopus cDNAs encoding CDC7-related kinases and suggested the possibility that chromosomal replication of higher eukaryotes may be regulated through conserved mechanisms involving Cdc7-related kinases. Here we report a murine cDNA and gene (muCdc7) encoding a serine/threonine kinase related to CDC7. The predicted coding frame for the longest cDNA for muCdc7 consists of 564 amino acids, which shares 46, 77, and 93% identity, respectively, with those of budding yeast, Xenopus, and human in kinase conserved domains. The chromosomal gene for muCdc7, located at the band 5E5 on the mouse chromosome 5, consists of 12 exons, and its exon/intron organization shares some similarity with that of other protein kinases including Cdk and cAMP-dependent kinase. Transcription of muCdc7, initiated at multiple sites over the 370-base pair promoter region, is repressed in the resting state and is induced at the G(1)/S boundary after growth factor stimulation in a growth factor-dependent cell line. Transient transfection assays indicated that a 231-base pair segment of the muCdc7 promoter containing three putative E2F binding sites and one Sp1 site but lacking TATA sequence is sufficient for response to growth stimulation.