Conjugated superoxide dismutase reduces extent of caudate injury after transient focal ischemia in cats.
Conjugated superoxide dismutase reduces extent of caudate injury after transient focal ischemia in cats.
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DOI：
10.1161/01.str.22.9.1193
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发表时间：
1991-09
期刊：
影响因子：
8.3
通讯作者：
Naoki Matsumiya;R. Koehler;J. Kirsch;R. Traystman
中科院分区：
文献类型：
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作者：
Naoki Matsumiya;R. Koehler;J. Kirsch;R. Traystman
我们在局灶性脑缺血再灌注模型中测试了30,000单位聚乙二醇偶联超氧化物歧化酶对缺血前和缺血后全身治疗的疗效。41只猫麻醉后，阻断左大脑中动脉和颈总动脉2小时，再灌注4小时。猫被随机分为三组：给药（10%聚乙二醇盐水，n = 17），缺血前3小时给药（n = 12），再灌注时给药（n = 12）。通过2,3,5-三苯四氮氯化染色计算缺血损伤的大小。与对照组（56 +/- 8%）相比，预处理组尾状核损伤显著减少（同侧尾状核体积的28 +/- 6%，平均+/- SEM）。后处理没有显著改善尾状核损伤（46 +/- 10%）。在缺血的第1小时和第2小时之间，用微球测定的同侧尾状动脉血流量在预处理时从11 +/- 4显著增加到16 +/- 5 ml/min/100 g，但在整个缺血过程中，用载药（8 +/- 2 ml/min/100 g）和处理后（10 +/- 3 ml/min/100 g）血流量保持不变。皮质损伤的大小（对照，17 +/- 5%；预处理，11 +/- 3%；处理后，17 +/- 5%的半球体积）各组间无显著差异。体感诱发电位恢复在各组间无差异。我们得出结论，在局灶性缺血再灌注模型中，共轭超氧化物歧化酶预处理可以改善尾状核等动脉末端区域的损伤程度。（摘要删节250字）
We tested the efficacy of preischemic and postischemic systemic treatment with 30,000 units polyethylene glycol-conjugated superoxide dismutase in a reperfusion model of focal cerebral ischemia. Forty-one anesthetized cats underwent 2 hours' occlusion of the left middle cerebral artery and both common carotid arteries followed by 4 hours of reperfusion. Cats were blindly assigned to one of three groups: treatment with vehicle (10% polyethylene glycol in saline, n = 17), pretreatment with drug 3 hours before ischemia (n = 12), and posttreatment with drug at the time of reperfusion (n = 12). Size of the ischemic injury was calculated from 2,3,5-triphenyltetrazolium chloride staining. Injury in the caudate nucleus was significantly reduced with pretreatment (28 +/- 6% of ipsilateral caudate volume, mean +/- SEM) compared with the vehicle (56 +/- 8%). Posttreatment did not significantly ameliorate caudate injury (46 +/- 10%). Between the first and second hours of ischemia ipsilateral caudate blood flow determined using microspheres increased significantly from 11 +/- 4 to 16 +/- 5 ml/min/100 g with pretreatment, but blood flow remained constant throughout ischemia with vehicle (8 +/- 2 ml/min/100 g) and posttreatment (10 +/- 3 ml/min/100 g). The size of cortical injury (vehicle, 17 +/- 5%; pretreatment, 11 +/- 3%; posttreatment, 17 +/- 5% of hemispheric volume) did not differ significantly among groups. Somatosensory evoked potential recovery did not differ among groups. We conclude that pretreatment with conjugated superoxide dismutase can ameliorate the extent of injury in an end-artery region, such as the caudate nucleus, in a reperfusion model of focal ischemia.(ABSTRACT TRUNCATED AT 250 WORDS)