Molecular cloning of the human Nurr1 gene: characterization of the human gene and cDNAs
Molecular cloning of the human Nurr1 gene: characterization of the human gene and cDNAs
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DOI：
10.1016/s0378-1119(99)00065-7
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发表时间：
1999-04-16
期刊：
影响因子：
3.5
通讯作者：
Nagatsu, T
中科院分区：
文献类型：
--
作者：
Ichinose, H;Ohye, T;Nagatsu, T
Nurr 1是转录因子核受体超家族的成员，主要在中枢神经系统中表达，包括发育中的多巴胺能神经元。最近，研究表明Nurr 1对中脑多巴胺能细胞分化至关重要。为了研究Nurr 1与帕金森病或其他神经精神疾病的发病机制的可能关系，我们克隆了人类Nurr 1基因并对其进行了表征。该基因在人类基因组中以单拷贝存在，包含8个外显子，跨度为8 kb。我们确定了该基因的完整核苷酸序列和侧翼区。潜在的调控区域包括NF-κ B、CREB和Sp1的共有结合位点。从胎儿脑中分离出的人Nurr 1 cDNA表明在人脑中存在Nurr 1的新剪接变体。(C)1999 Elsevier Science B. V.保留所有权利。
Nurr1 is a member of the nuclear receptor superfamily of transcription factors that is expressed predominantly in the central nervous system, including developing dopaminergic neurons. Recently, it was demonstrated that Nurr1 is critical for midbrain dopaminergic cell differentiation. In order to investigate a possible relation of Nurr1 with the pathogenesis of Parkinson's disease or other neuropsychiatric disorders, we have cloned and characterized the human Nurr1 gene. The gene exists as a single copy in the human genome and comprises eight exons spanning 8 kb. We determined the complete nucleotide sequence and flanking regions of the gene. Potential regulatory regions included consensus binding sites for NF-kappa B, CREB, and Sp1. Isolation of human Nurr1 cDNAs from fetal brain suggested the presence of a new splicing variant of Nurr1 in the human brain. (C) 1999 Elsevier Science B.V. All rights reserved.